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Advances in Stem Cell Research pp 227–243Cite as

Omics in Stem Cell Therapy: The Road Ahead

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Understanding self-renewal and differentiation of stem cells at molecular levels is essential for both basic research and clinical applications of stem cells. The complexity of stem cell fate is controlled by the sophisticated mechanisms of temporal and spatial gene expression and protein function. Thus, the capacity to control a gene’s expression, build cellular reporters that reflect the activity state of lineage-specific endogenous genes, and either create or repair disease alleles is essential for realizing the potential of stem cell technology. In this chapter, we introduce zinc finger nucleases (ZFNs) as a tool to efficiently and selectively manipulate a given gene at the endogenous locus for advancing both our understanding as well as therapeutic application of stem cell biology. Proteins too are key players in the cell and often serve as biomarkers. They have diverse features that are not predictable from gene sequences or from the level of transcripts. Major breakthroughs in stem cell research were made by the identification of protein biomarkers such as colony-stimulating factors (CSFs) and cell-surface CD molecules. Recently, the Human Proteome Organization (HUPO) and Asia Oceanic HUPO (AOHUPO) launched initiatives for systematic MS-based proteomics as well as a gene coded proteomic approach to understanding stem cell differentiation. These studies will shed new light on stem cell biology and accelerate clinical applications of stem cells.

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Acknowledgments

The authors thank Mrs. Jee Hyun Oh, Mr. Daesik Kim, Ms. Jin-A Kang, and Dr. Enkhjaigal Bayarsaikhan for their efforts. This research was supported by a grant from the National Research Foundation to KB (2011-0003677). Funding in part was also provided by a National Research Foundation (2010-0020573; BL) and the 21ST frontier science program SC-2110 of the Korea stem cell research center (BL).

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Authors and Affiliations

  1. Center for Genomics and Proteomics and Stem Cell Core Facility, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon, Korea

    Kyunghee Byun & Bonghee Lee

  2. Department of Anatomy and Cell Biology, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea

    Kyunghee Byun, Goo-Bo Jeong & Bonghee Lee

  3. Sigma-Aldrich, Inc., St. Louis, MO, USA

    Trevor N. Collingwood

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  1. Kyunghee Byun
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  2. Goo-Bo Jeong
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  3. Trevor N. Collingwood
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  4. Bonghee Lee
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Correspondence to Bonghee Lee .

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Editors and Affiliations

  1. Royan Institute, Department of Developmental Biology, University of Science and Culture, PO Box 19395-4644, Tehran, Iran

    Hossein Baharvand

  2. , Regenerative Medicine, Royan Institute for Stem Cell Biology an, P.O. Box: 19395-4644, Tehran, Iran

    Nasser Aghdami

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Byun, K., Jeong, GB., Collingwood, T.N., Lee, B. (2012). Omics in Stem Cell Therapy: The Road Ahead. In: Baharvand, H., Aghdami, N. (eds) Advances in Stem Cell Research. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-940-2_13

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