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Studies on Men's Health and Fertility pp 257–273Cite as

Methods for the Detection of ROS in Human Sperm Samples

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Abstract

Male-factor significantly contributes to infertility in couples of reproductive age. Several studies have shown that oxidative stress (OS) is involved in the pathophysiology of male infertility. It is caused by an imbalance between the formation of reactive oxygen species (ROS) and the ability of the antioxidants to scavenge them. There are several methods to measure seminal ROS in the clinical setting, most notably among them is the chemiluminescence assay. This technique measures the global ROS, i.e., both the intra- and extracellular ROS. The measurement of seminal ROS employing the chemiluminescence technique in clinical andrology labs is explained in this chapter. Through a deeper understanding of ROS and its measurement, clinical andrology labs can better assist patients to achieve increased rates of fertility and pregnancy.

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Author information

Authors and Affiliations

  1. Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, 44195, USA

    David Benjamin BA, Rakesh K. Sharma PhD & Arozia Moazzam MD

  2. Center for Reproductive Medicine, Cleveland Clinic, Lerner College of Medicine, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

    Ashok Agarwal PhD

Authors
  1. David Benjamin BA
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  2. Rakesh K. Sharma PhD
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  3. Arozia Moazzam MD
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  4. Ashok Agarwal PhD
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Corresponding author

Correspondence to Ashok Agarwal PhD .

Editor information

Editors and Affiliations

  1. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

  2. Faculty of Science and IT, Department of Biological Sciences, University of Newcastle, University Drive 1, Callaghan, 2308, New South Wales, Australia

    R. John Aitken

  3. , Department of Male Infertility, Centro Androgen, C/FERNANDO MACIAS 8, 1ºC, La Coruña, 15004, La Coruña, Spain

    Juan G. Alvarez

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Benjamin, D., Sharma, R.K., Moazzam, A., Agarwal, A. (2012). Methods for the Detection of ROS in Human Sperm Samples. In: Agarwal, A., Aitken, R., Alvarez, J. (eds) Studies on Men's Health and Fertility. Oxidative Stress in Applied Basic Research and Clinical Practice. Humana Press. https://doi.org/10.1007/978-1-61779-776-7_13

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