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Nuclear Reprogramming and Stem Cells pp 141–153Cite as

Noncell Autonomous Reprogramming to a Pluripotent State

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

In 2006, Takahashi and Yamanaka discovered that somatic cells could be reprogrammed to a pluripotent state by the expression of a defined set of exogenous transcription factors. This represents a significant breakthrough for the practical use of stem cells in regenerative medicine. Since then, several strategies have been used to generate induced pluripotent (iPS) cells that include nucleic acid and nonnucleic acid-based approaches, with and without epigenetic modifications. The purpose of these different approaches for generating iPS cells, besides understanding the ­underlying mechanism, is to develop a facile method for reprogramming without genetic alteration, suitable for clinical use. Here, we discuss different strategies for generating iPS cells, with an emphasis on a recent noncell autonomous approach to reprogram somatic progenitors that regenerate cornea to a pluripotent state through the recruitment of endogenous transcription factors.

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Author information

Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE, USA

    Sowmya Parameswaran & Sudha Balasubramanian

  2. Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    Iqbal Ahmad

Authors
  1. Sowmya Parameswaran
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  2. Sudha Balasubramanian
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  3. Iqbal Ahmad
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Corresponding author

Correspondence to Iqbal Ahmad .

Editor information

Editors and Affiliations

  1. Leeds Institute of Genetics,, Health & Therapeutics, University of Leeds, Leeds, LS2 9JT, United Kingdom

    Justin Ainscough

  2. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Shinya Yamanaka

  3. Inst. Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Kyoto, 606-8507, Japan

    Takashi Tada

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Parameswaran, S., Balasubramanian, S., Ahmad, I. (2011). Noncell Autonomous Reprogramming to a Pluripotent State. In: Ainscough, J., Yamanaka, S., Tada, T. (eds) Nuclear Reprogramming and Stem Cells. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-225-0_12

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