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Stem Cells and Cancer pp 127–140Cite as

Mouse Mammary Tumor Virus: Stem Cells and Mammary Cancer

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Part of the book series: Cancer Drug Discovery and Development ((CDD&D))

Abstract

The paradigm of mammary cancer induction by the mouse mammary tumor virus (MMTV) is used to illustrate the body of evidence that supports the hypothesis that mammary epithelial stem/progenitor cells represent targets for oncogenic transformation. It is argued that this is not a special case applicable only to MMTV-induced mammary cancer, because MMTV acts as an environmental mutagen producing random interruptions in the somatic DNA of infected cells by insertion of proviral DNA copies. In addition to disrupting the host genome, the proviral DNA also influences gene expression through its associated enhancer sequences over significant intergenomic distances. Genes commonly affected by MMTV insertion in multiple individual tumors include, the Wnt genes, the FGF gene family, and the Notch gene family. All of these gene families are known to play essential roles in stem cell maintenance and behavior in a variety of organs. The MMTV-induced mutations accumulate in cells that are long lived and possess the properties of stem cells, namely, self-renewal and the capacity to produce divergent epithelial progeny through asymmetric division. The evidence shows that epithelial cells with these properties are present in normal mammary glands, may be infected with MMTV, and become transformed to produce epithelial hyperplasia through MMTV-induced mutagenesis and progress to frank mammary malignancy. Retroviral marking via MMTV proviral insertion demonstrates that this process progresses from a single mammary epithelial cell that possesses all of the features ascribed to tissue-specific stem cells.

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Authors and Affiliations

  1. Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, Building 37, Room 1106, 37 Convent Drive, Bethesda, MD, 20892, USA

    Gilbert H. Smith

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  1. Gilbert H. Smith
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Smith, G.H. (2009). Mouse Mammary Tumor Virus: Stem Cells and Mammary Cancer. In: Teicher, B., Bagley, R. (eds) Stem Cells and Cancer. Cancer Drug Discovery and Development. Humana Press. https://doi.org/10.1007/978-1-60327-933-8_10

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