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Epithelial Calcium Transport in Crustaceans: Adaptation to Intrinsic and Extrinsic Stressors

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Epithelial Transport Physiology

Abstract

Since the classical studies of Ussing employing a nonmammalian isolated epithelium (frog skin) to explore the basic principles of ion transport, physiologists have adopted increasingly reductionist approaches to dissect the biophysical mechanisms undergirding biological transport. In vitro characterization has employed isolated perfused organs, isolated epithelia, and reconstituted vesicle studies. Depth of resolution has been further enhanced by the emerging molecular revolution. Following years of deconstruction, physiologists are now engaging in reconstruction, namely putting the genes back into the organism. This contribution attempts such an integrative approach for a single electrolyte, calcium (Ca2+), in a nonmammalian epithelium, the crayfish antennal gland (kidney). Two collaborating laboratories have archived an inventory of Ca2+ associated proteins believed to play a role in transcellular Ca2+ movement. Using the basic building blocks (expression profiles of key Ca2+ associated proteins and their regulators), the authors attempt to reconstruct a whole cell model for Ca2+ regulation in transporting epithelium (compared with a nonepithelial tissue) under stressors that perturb Ca2+ homeostasis which originate either intrinsically (the postmolt stage of the molting cycle) or extrinsically (unanticipated cold acclimation). Through horizontal integration of expression profiles of seven target Ca2+ associated proteins in epithelial and nonepithelial tissue under two contrasting experimental conditions, emergent themes inform the physiological complexity of Ca2+ homeostasis. Integration at the next level will require placing the epithelium in the context of organismic Ca2+ balance. The unique Ca2+ handling capabilities of the freshwater crayfish make it an excellent nonmammalian model for those studies.

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Acknowledgments

The research reported from the authors’ laboratories at Wright State University and Kenyon College has been supported by National Science Foundation grant number IBN 0445202 (to M.W., Y.G., and C.G.). The original data on cold acclimation resulted from the thesis research of two master’s students, Daniel Whalen and Zeenat Jamal (assisted by undergraduates and interns Francieli Vigo and Ashkahn Golshani).

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Authors and Affiliations

  1. Department of Biological Sciences, College of Science and Mathematics, Wright State University, 45435, Dayton, OH, USA

    Michele G. Wheatly

  2. Department of Biological Sciences, Wright State University, Dayton, OH, USA

    Yongping Gao Ph.D

  3. Department of Biology, Kenyon College, Gambier, OH, USA

    Christopher M. Gillen Ph.D

Authors
  1. Michele G. Wheatly
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  2. Yongping Gao Ph.D
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  3. Christopher M. Gillen Ph.D
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Corresponding author

Correspondence to Michele G. Wheatly .

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Editors and Affiliations

  1. College of Medicine, University of Florida, SW. Archer Road 1600, Gainesville, 32610-0274, U.S.A.

    George A. Gerencser

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Wheatly, M.G., Gao, Y., Gillen, C.M. (2010). Epithelial Calcium Transport in Crustaceans: Adaptation to Intrinsic and Extrinsic Stressors. In: Gerencser, G. (eds) Epithelial Transport Physiology. Humana Press. https://doi.org/10.1007/978-1-60327-229-2_4

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