Abstract
The objective of this study was to covalently immobilize Candida antarctica type B lipase (CALB) onto silanized green coconut fibers. Variables known to control the number of bonds between enzyme and support were evaluated including contact time, pH, and final reduction with sodium borohydride. Optimal conditions for lipase immobilization were found to be 2h incubation at both pH 7.0 and 10.0. Thermal stability studies at 60°C showed that the immobilized lipase prepared at pH 10.0 (CALB-10) was 363-fold more stable than the soluble enzyme and 5.4-fold more stable than the biocatalyst prepared at pH 7.0 (CALB-7). CALB-7 was found to have higher specific activity and better stability when stored at 5°C. When sodium borohydride was used as reducing agent on CALB-10 there were no improvement in storage stability and at 60°C stability was reduced for both CALB-7 and CALB-10.
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References
Jaeger, K. and Reetz, M. T. (1998), Trends Biotechnol. 16, 396–403.
Dalla-Vecchia, R., Nascimento, M. G., and Soldi, V. (2004), Quím. Nova. 27, 623–630.
Sharma, R., Chisti, Y., and Banerjee, U. C. (2001), Biotechnol. Adv. 19, 627–662.
Villeneuve, P., Muderhwa, J. M., Graille, J., and Haas, M. J. (2000), J. Mol. Catal. B: Enzyme, 9, 113–148.
Stark, M.-B. and Holmberg, K. (1989), Biotechnol. Bioeng. 34, 942–950.
Tardioli, P. W., Fernandez-Lafuente, R., Guisan, J. M., and Giordano, R. L. (2003), Biotechnol. Prog. 19, 564–574.
Kennedy, J. F., White, C. A., and Melo, E. H. M. (1988), Chimicaoggi. 5, 21–29.
Fernández-Lafuente, R., Rodríguez, V., Mateo, C., et al. (1999), J. Mol. Catal. B: Enzyme 7, 181–189.
Mateo, C., Abian, O., Fernández-Lafuente, R., and Guisán, J. M. (2000), Enzyme Microb. Tech. 26, 509–515.
Blanco, R. M., Calvete, J. J., and Guisán, J. M. (1989), Enzyme Microb. Technol. 11, 353–359.
Dey, G., Nagpal, V., and Banerjee, R. (2002), Appl. Biochem. Biotechnol. 102–103, 303–313.
D’Souza, S. F. and Godbole, S. S. (2002), J. Biochem. Biophys. Methods 52, 59–62.
Castro, H. F., Lima, R., and Roberto, I. C. (2001), Biotechnol. Prog. 17, 1061–1064.
Rosa, M. F., Bezerra, F. C., Brígida, A. I. S., and Brígido, A. K. L. (2002), Aproveitamento de resíduos da indústria da água de coco verde como substrato agrícola: 1—Processo de obtenção. Proceedings of the VI Seminário Nacional de Residuos, Gramado, Brazil (in Portugese).
Cardias, H. C. T., Grininger, C. C., Trevisan, H. C., Guisan, J. M., and Giordano, R. L. C. (1999), Braz. J. Chem. Eng. 16, 141–148.
Guisan, J. M., Bastida, A., Cuesta, C., Fernandez-Lafuente, R., and Rossel, C. M. (1991), Biotechnol. Bioeng. 38, 1144–1152.
Adriano, W. S., Costa-Filho, E. H., Silva, J. A., Giordano, R. L. C., and Goncalves, L. R. B. (2005), Braz. J. Chem. Eng. 22, 529–538.
Pereira, G. H. A., Guisán, J. M., and Giordano, R. L. C. (1997), Braz. J. Chem. Eng. 14, 327–332.
Rodrigues, D. S. (2005), Master Thesis, Estudo da imobilização de lipase B de Candida antarctica em carvão i quitorana (in Portugese). Universidade Federal do Ceará, Fortaleza, Brazil.
Castro, H. F., Silva, M. L. C. P., and Silva, G. L. J. P. (2000), Braz. J. Chem. Eng. 17, 849–857.
Soares, C. M. F., Castro, H. F., Santana, M. H. A., and Zanin, G. M. (2002), Appl. Biochem. Biotechnol. 98–100, 863–874.
Pino, G. A. H. (2005), Master Thesis, Pontificia Universidade Católica do Rio de Janeiro, Rio de Janeiro, Brazil.
Gomes, F. M., Silva, G. S., Pinatti, D. G., Conte, R. A., and Castro, H. F. (2005), Appl. Biochem. Biotechnol. 121, 255–268.
Palomo, J. M., Muñoz, G., Fernándes-Lorente, G., et al. (2003), J. Mol. Catal. B: Enzyme 21, 201–210.
Otero, C., Ballesteros, A., and Guisan, J. M. (1988), Appl. Biochem. Biotechnol. 19, 163–175.
Pedroche, J., Yust, M. M., Mateo, C., et al. (2007), Enzyme Microb. Technol in press.
Isgrove, F. H., Williams, R. J. H., Niven, G. W., and Andrews, A. T. (2001), Enzyme Microb. Technol. 28, 225–232.
Mateo, C., Torres, R., Fernandez-Lorente, G., et al. (2003), Biomacromolecules 4, 772–777.
Mateo, C., Palomo, J. M., Fuentes, M., et al. (2006), Enzyme Microb. Technol. 39, 274–280.
Blanco, R. M. and Guisán, J. M. (1989), Enzyme Microb. Technol. 11, 360–366.
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Brígida, A.I.S., Pinheiro, Á.D.T., Ferreira, A.L.O., Pinto, G.A.S., Gonçalves, L.R.B. (2007). Immobilization of Candida antarctica Lipase B by Covalent Attachment to Green Coconut Fiber. In: Mielenz, J.R., Klasson, K.T., Adney, W.S., McMillan, J.D. (eds) Applied Biochemistry and Biotecnology. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-60327-181-3_7
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DOI: https://doi.org/10.1007/978-1-60327-181-3_7
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