Abstract
This chapter describes the development and use of suspension antibody microarrays for protein profiling in several human body fluids. In suspension microarrays, which allow the simultaneous analysis of a variety of analytes within a single experiment, capture antibodies are coupled onto color-coded microspheres. Applications are described, with emphasis on analyses of proteins present in different types of body fluids, like serum or plasma, tears, cerebrospinal, pleural, and synovial fluids, and cell culture supernatants. The chapter is divided into the generation of suspension microarrays, sample preparation, processing, and validation of analytical performance.
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© 2007 Humana Press Inc., Totowa, NJ
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Wittemann, S., Eisinger, D.P., Stephen, L.L., Joos, T.O. (2007). Multiplexed Immunoassays for Protein Profiling in Human Body Fluids. In: Thongboonkerd, V. (eds) Proteomics of Human Body Fluids. Humana Press. https://doi.org/10.1007/978-1-59745-432-2_3
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DOI: https://doi.org/10.1007/978-1-59745-432-2_3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-657-3
Online ISBN: 978-1-59745-432-2
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