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Gene Expression Profiling in Leiomyoma in Response to GnRH Therapy and TGF-β

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Genomics in Endocrinology

Part of the book series: Contemporary Endocrinology ((COE))

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Abstract

Microarray technology has proven to be a powerful tool for simultaneous profiling of the expression of a large number of genes in various cells and tissues under normal physiological or pathological conditions. The introduction of microarray in profiling the pattern of gene expression in various female reproductive tract tissues and cells resulted in the generation of a large body of new information representing their molecular environments under both normal physiological and pathological conditions. The identification of transcripts for many genes, whose products’ functional relevance in reproductive tissues was not previously predicated, has opened new research opportunities in the field of reproductive endocrinology. Here, we shall discuss the application of microarray in studying gene expression profiling in myometrium and leiomyoma and the consequence of GnRHa therapy on their expression, as well as their profiles in smooth muscle cells isolated from these tissues in response to direct action of GnRHa. We shall also discuss the influence of transforming growth factor beta (TGF-β), a profibrotic cytokine, on leiomyoma and myometrial smooth muscle gene expression profile and the consequence of TGF-β receptor type II antisense treatment as an alternative approach to suppress leiomyoma growth. Furthermore, we shall discuss the experimental design with appropriate steps and procedures for RNA preparation, microarray hybridization, image acquisition and data analysis, interpretation, confirmation, and finally the biological significance of these genes.

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Authors
  1. Nasser Chegini PhD
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  2. Xiaoping Luo MD
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Editors and Affiliations

  1. Cincinnati Children’s Hospital Medical Center (CCHMC), University of Cincinnati College of Medicine, 3333 Burnett Avenue, Cincinnati, OH 45229, USA

    Stuart Handwerger MD

  2. University of Cincinnati College of Medicine, Cincinnati, OH, USA

    Bruce Aronow PhD

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© 2008 Humana Press

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Chegini, N., Luo, X. (2008). Gene Expression Profiling in Leiomyoma in Response to GnRH Therapy and TGF-β. In: Handwerger, S., Aronow, B. (eds) Genomics in Endocrinology. Contemporary Endocrinology. Humana Press. https://doi.org/10.1007/978-1-59745-309-7_4

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  • DOI: https://doi.org/10.1007/978-1-59745-309-7_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-651-1

  • Online ISBN: 978-1-59745-309-7

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