Abstract
The dermatophytes are a specialized group of fungi which infect the keratinized tissues of humans (hair, nails, and skin) and cause superficial infections. While several studies have been conducted to develop methods to determine the susceptibilities of yeast and filamentous fungi, similar studies for dermatophytes have only recently taken place. In this chapter, we will review how susceptibility testing of dermatophytes was developed and how it has already been applied to clinical samples. With several agents now available for treating infections due to dermatophytes, susceptibility testing will serve as a valuable tool for clinicians as they choose the most appropriate treatment option. Studies are still needed to establish interpretive breakpoints for antifungal agents used in the treatment of superficial fungal infections.
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References
CLSI (2008) Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard – third edition. CLSI document M27-A3. Clinical and Laboratory Standards Institute, CLSI, 940 West Valley Road, Suite 1400, Wayne, PA 19087–1898 USA
CLSI (2008) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard- second edition. CLSI document M38-A2 [ISBN 1–56238–668–9]. CLSI, 940 West Valley Road, Suite 1400, Wayne, PA 19087–1898 USA
Weitzman I, Summerbell RC (1995) The dermatophytes. Clin Microbiol Rev 8(2):240–259
Ghannoum MA, Chaturvedi V, Espinel-Ingroff A et al (2004) Intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes. J Clin Microbiol 42(7):2977–2979
Ghannoum MA, Rex JH, Galgiani JN (1996) Susceptibility testing of fungi: current status and correlation of in vitro data with clinical outcome. J Clin Microbiol 34(3):489–495
Rex JH, Pfaller MA, Walsh TJ et al (2001) Antifungal susceptibility testing: practical aspects and current challenges. Clin Microbiol Rev 14(4):643–658
Norris HA, Elewski BE, Ghannoum MA (1999) Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method. J Am Acad Dermatol 40(6):S9–S13
Jessup CJ, Warner J, Isham N, Hasan I, Ghannoum MA (2000) Antifungal susceptibility testing of dermatophytes: establishing a medium for inducing conidial growth and evaluation of susceptibility of clinical isolates. J Clin Microbiol 38(1):341–344
Ghannoum MA, Chaturvedi V, Espinel-Ingroff A et al. (submitted) An interlaboratory QC guidelines study for a proposed antifungal susceptibility method for the testing of dermatophytes, modified from CLSI M38-A. J Clin Microbiol
Bradley MC, Leidich S, Isham N, Elewski BE, Ghannoum MA (1999) Antifungal susceptibilities and genetic relatedness of serial Trichophyton rubrum isolates from patients with onychomycosis of the toenail. Mycoses 42(suppl 2):105–110
Ghannoum MA, Isham N, Hajjeh R et al (2003) Tinea capitis in Cleveland: survey of elementary school students. J Am Acad Dermatol 48:189–193
Ghannoum MA, Hajjeh RA, Scher R et al (2000) A large-scale North American study of fungal isolates from nails: frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 43:641–648
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Chand, D.V., Ghannoum, M.A. (2012). Susceptibility Testing of Dermatophytes. In: Hall, G. (eds) Interactions of Yeasts, Moulds, and Antifungal Agents. Humana Press. https://doi.org/10.1007/978-1-59745-134-5_4
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DOI: https://doi.org/10.1007/978-1-59745-134-5_4
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