Abstract
Since somatic cell hybrids between rodent cells derived from continuous cell lines and human cells lose (segregate) human chromosomes (Weiss and Green, 1967), it becomes possible to map human genes to specific human chromosomes (Ruddle, 1973) by determining whether the expression of a given human phenotype segregates concordantly with the presence of a specific human chromosome in the hybrid cells. Cells derived from different mammalian species, including man (Girardi et al., 1965), can be transformed by the small DNA tumor virus, simian virus 40 (SV40). The SV40 genome becomes covalently linked to cellular DNA in these cells (Sambrook et al., 1968), which also express a nuclear tumor (T) antigen (Black. et al., 1963), apparently coded by a viral gene (Tegtmeyer, 1974), and a tumor specific transplantation antigen on the cell surface (Habel, 1965). Fusion of T antigen-positive with T antigen-negative cells results in the production of heterokaryons which express T antigen in the nuclei derived from both parent cells (Steplewski et. al., 1968) and in the production of T antigenpositive and negative hybrid cells (Weiss, 1970).
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© 1977 Plenum Press, New York
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Croce, C.M. (1977). Mapping the SV40 Integration Sites in SV40-Transformed Human Cells. In: Nichols, W.W., Murphy, D.G. (eds) Senescence. Cellular Senescence and Somatic Cell Genetics, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2508-6_8
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DOI: https://doi.org/10.1007/978-1-4684-2508-6_8
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