In Situ Hybridization Histochemistry as a Supplement to Immunohistochemistry

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Abstract

Immunohistochemistry has proven to be an invaluable approach in the localization of peptidergic as well as classical transmitter compounds, their synthetic enzymes and other cell specific antigens in brain tissue. Its extensive use over the past decade has allowed the definition of various anatomical circuits with a high degree of resolution at both the light and ultrastructural levels. Despite the tremendous advances that have been made in neuroanatomical studies employing immunocytochemistry, there are still two major limitations in the use of this technique. These pertain to the nonquantifiable nature of the procedure and the frequent occurrence of both false positive and false negative results in staining.