Abstract
Our chelate sensor method for detecting mercury is based on the high affinity of thiol-containing ligands for the mercuric ion. The method combines the simple ELISA-format with the selective complexation of mercuric ions by dithiocarbamate chelators. The first assay uses a sandwich chelate formed by a ligand immobilized on the well of an ELISA plate, mercuric ion of the analyzed sample, and another ligand bound to the reporter enzyme. Our second assay utilizes competition between the binding of mercuric ions and an organomercury-conjugate to a chelating conjugate. Specifically, it involves a chelator doped on the solid surface and a mercury-containing enzyme-tracer. Both assays were further characterized by testing them under several conditions that might be encountered during some practical applications of the assay. Finally, they were tested with river water and human saliva to yield a good correlation with spike levels.
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Kido, H., Szurdoki, F., Gustin, M.S., Hammock, B.D. (1999). Chelate Sensor Method for Mercury. In: Sarkar, B. (eds) Metals and Genetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4723-5_7
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DOI: https://doi.org/10.1007/978-1-4615-4723-5_7
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