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Biotechnology of Crucifers pp 13–22Cite as

Molecular Cytogenetics

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Abstract

Cytogenetics has played a key role in the history of scientific research in the Brassicaceae since the start of the last century. The discovery of the Brassica “U’s Triangle” species, elucidation of phylogenetic relationships and investigations of chromosome evolution all contributed to building up the basic genomic understanding of the Brassicaceae we have today. The advent of molecular cytogenetics in this family in the last 20 years has led to a progressively greater understanding of the factors underlying chromosome dynamics and organisation, meiotic and mitotic mechanisms and cell division processes. In addition, linking molecular cytogenetics with other molecular techniques, such as marker studies, DNA sequencing and protein expression analysis, has bridged the gap between chromosomes and linkage groups, resulting in a wealth of new information in this family. Future prospects for molecular cytogenetics in the Brassicaceae are bright. The recent and imminent release of additional Brassicaceae genomes will greatly facilitate development of probes for fluorescent in situ hybridisation as well as a comprehensive understanding of gene expression and protein interactions during cell division.

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References

  • Alix K, Joets J, Ryder CD, Moore G, Barker G, Bailey JP et al (2008) The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation. Plant J 56:1030–44

    Article  PubMed  CAS  Google Scholar 

  • Armstrong SJ, Jones GH (2003) Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana. J Exp Bot 54(380):1–10

    Article  PubMed  CAS  Google Scholar 

  • Chen HF, Wang H, Li ZY (2007) Production and genetic analysis of partial hybrids in intertribal crosses between Brassica species (B. rapa, B. napus) and Capsella bursa-pastoris. Plant Cell Rep 26(10):1791–800

    Article  PubMed  CAS  Google Scholar 

  • Chen S, Nelson MN, Chèvre AM, Jenczewski E, Li Z, Mason AS et al (2011) Trigenomic bridges for Brassica improvement. Critical Reviews in Plant Science 30(6):524–47

    Article  CAS  Google Scholar 

  • Comai L, Tyagi AP, Lysak MA (2003) FISH analysis of meiosis in Arabidopsis allopolyploids. Chromosome Res 11(3):217–26

    Article  PubMed  CAS  Google Scholar 

  • Dhar MK, Kaul S, Kour J (2011) Towards the development of better crops by genetic transformation using engineered plant chromosomes. Plant Cell Rep 30(5):799–806

    Article  PubMed  CAS  Google Scholar 

  • Du XZ, Ge XH, Zhao ZG, Li ZY (2008) Chromosome elimination and fragment introgression and recombination producing intertribal partial hybrids from Brassica napus × Lesquerella fendleri crosses. Plant Cell Rep 27(2):261–71

    Article  PubMed  CAS  Google Scholar 

  • Figueroa DM, Bass HW (2010) A historical and modern perspective on plant cytogenetics. Brief Funct Genomics 9(2):95–102

    Article  PubMed  Google Scholar 

  • FitzJohn RG, Armstrong TT, Newstrom-Lloyd LE, Wilton AD, Cochrane M (2007) Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape. Euphytica 158(1–2):209–30

    Article  Google Scholar 

  • Frandsen HN, Winge O (1932) Brassica napocampestris, a new constant amphidiploid species hybrid. Hereditas 16:212–8

    Article  Google Scholar 

  • Harberd DJ, McArthur ED (1980) Meiotic analysis of some species and genus hybrids in the Brassiceae. In: Tsunoda S, Hinata K, Gomez-Campo C (eds) Brassica crops and wild allies: biology and breeding. Japan Scientific Societies, Tokyo, pp 65–87

    Google Scholar 

  • Hasterok R, Ksiazczyk T, Wolny E, Maluszynska J (2005) FISH and GISH analysis of Brassica genomes. Acta Biol Cracov Bot 47(1):185–92

    Google Scholar 

  • Heneen WK, Geleta M, Brismar K, Xiong ZY, Pires JC, Hasterok R et al (2012) Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa-B. oleracea monosomic alien addition lines. Ann Bot 109(7):1227–42

    Article  PubMed  CAS  Google Scholar 

  • Heslop-Harrison JS, Murata M, Ogura Y, Schwarzacher T, Motoyoshi F (1999) Polymorphisms and genomic organization of repetitive DNA from centromeric regions of Arabidopsis chromosomes. Plant Cell 11(1):31–42

    PubMed  CAS  Google Scholar 

  • Howell EC, Barker GC, Jones GH, Kearsey MJ, King GJ, Kop EP et al (2002) Integration of the cytogenetic and genetic linkage maps of Brassica oleracea. Genetics 161(3):1225–34

    PubMed  CAS  Google Scholar 

  • Iwabuchi M, Itoh K, Shimamoto K (1991) Molecular and cytological characterization of repetitive DNA sequences in Brassica. Theor Appl Genet 81(3):349–55

    Article  CAS  Google Scholar 

  • Jenczewski E, Eber F, Grimaud A, Huet S, Lucas MO, Monod H et al (2003) PrBn, a major gene controlling homeologous pairing in oilseed rape (Brassica napus) haploids. Genetics 164(2):645–53

    PubMed  CAS  Google Scholar 

  • Kato A, Lamb JC, Birchler JA (2004) Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci U S A 101(37):13554–9

    Article  PubMed  CAS  Google Scholar 

  • Kim H, Choi SR, Bae J, Hong CP, Lee SY, Hossain MJ et al (2009) Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa. BMC Genomics 10:432

    Article  PubMed  Google Scholar 

  • Koornneef M, Fransz P, de Jong H (2003) Cytogenetic tools for Arabidopsis thaliana. Chromosome Res 11(3):183–94

    Article  PubMed  CAS  Google Scholar 

  • Lagercrantz U, Lydiate DJ (1996) Comparative genome mapping in Brassica. Genetics 144:1903–10

    PubMed  CAS  Google Scholar 

  • Leflon M, Eber F, Letanneur JC, Chelysheva L, Coriton O, Huteau V et al (2006) Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids. Theor Appl Genet 113:1467–80

    Article  PubMed  CAS  Google Scholar 

  • Leflon M, Grandont L, Eber F, Huteau V, Coriton O, Chelysheva L et al (2010) Crossovers get a boost in Brassica allotriploid and allotetraploid hybrids. Plant Cell 22:2253–64

    Article  PubMed  CAS  Google Scholar 

  • Ma N, Li ZY, Cartagena JA, Fukui K (2006) GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus. Plant Cell Rep 25(10):1089–93

    Article  PubMed  CAS  Google Scholar 

  • Maluszynska J, Heslop-Harrison JS (1993) Physical mapping of rDNA loci in Brassica species. Genome 36(4):774–81

    Article  PubMed  CAS  Google Scholar 

  • Mason AS, Huteau V, Eber F, Coriton O, Yan G, Nelson MN et al (2010) Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids. Chromosome Res 18(6):655–66, Epub 22/6/2010

    Article  PubMed  CAS  Google Scholar 

  • Mizushima U (1980) Genome analysis in Brassica and allied genera. In: Tsunoda S, Hinata K, Gomez-Campo C (eds) Brassica crops and wild allies: biology and breeding. Japan Scientific Societies, Tokyo, pp 89–106

    Google Scholar 

  • Morinaga T (1934) Interspecific hybridisation in Brassica VI. The cytology of F1 hybrids of B. juncea and B. nigra. Cytologia 6:62–7

    Article  Google Scholar 

  • Murata M, Ogura Y, Motoyoshi F (1994) Centromeric repetitive sequences in Arabidopsis thaliana. Jpn J Genet 69(4):361–70

    Article  PubMed  CAS  Google Scholar 

  • Navabi ZK, Stead KE, Pires JC, Xiong Z, Sharpe AG, Parkin IAP et al (2011) Analysis of B-genome chromosome introgression in interspecific hybrids of Brassica napus × B. carinata. Genetics 187:659–73

    Article  PubMed  CAS  Google Scholar 

  • Nicolas SD, Leflon M, Liu Z, Eber F, Chelysheva L, Coriton O et al (2008) Chromosome ‘speed dating’ during meiosis of polyploid Brassica hybrids and haploids. Cytogenet Genome Res 120:331–8

    Article  PubMed  CAS  Google Scholar 

  • Nicolas SD, Leflon M, Monod H, Eber F, Coriton O, Huteau V et al (2009) Genetic regulation of meiotic cross-overs between related genomes in Brassica napus haploids and hybrids. Plant Cell 21:373–85

    Article  PubMed  CAS  Google Scholar 

  • Olin-Fatih M, Heneen WK (1992) C-banded karyotypes of Brassica campestris, Brassica oleracea, and Brassica napus. Genome 35(4):583–9

    Article  Google Scholar 

  • Parkin IAP, Sharpe AG, Lydiate DJ (2003) Patterns of genome duplication within the Brassica napus genome. Genome 46(2):291–303

    Article  PubMed  CAS  Google Scholar 

  • Rygulla W, Snowdon RJ, Eynck C, Koopmann B, von Tiedemann A, Lühs W et al (2007) Broadening the genetic basis of Verticillium longisporum resistance in Brassica napus by interspecific hybridization. Phytopathology 97:1391–6

    Article  PubMed  CAS  Google Scholar 

  • Saal B, Brun H, Glais I, Struss D (2004) Identification of a Brassica juncea-derived recessive gene conferring resistance to Leptosphaeria maculans in oilseed rape. Plant Breeding 123(6):505–11

    Article  CAS  Google Scholar 

  • Schelfhout CJ, Snowdon R, Cowling WA, Wroth JM (2006) Tracing B-genome chromatin in Brassica napus × B. juncea interspecific progeny. Genome 49:1490–7

    Article  PubMed  CAS  Google Scholar 

  • Snowdon R, Köhler W, Köhler A (1997a) Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes. Genome 40:582–7

    Article  PubMed  CAS  Google Scholar 

  • Snowdon RJ, Kohler W, Friedt W, Kohler A (1997b) Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids. Theor Appl Genet 95(8):1320–4

    Article  CAS  Google Scholar 

  • Speicher MR, Carter NP (2005) The new cytogenetics: blurring the boundaries with molecular biology. Nat Rev Genet 6(10):782–92

    Article  PubMed  CAS  Google Scholar 

  • Szadkowski E, Eber F, Huteau V, Lodé M, Huneau C, Belcram H et al (2010) The first meiosis of resynthesized Brassica napus, a genome blender. New Phytol 186:102–12

    Article  PubMed  CAS  Google Scholar 

  • Tu YQ, Sun J, Liu Y, Ge XH, Zhao ZG, Yao XC et al (2008) Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica. Plant Cell Rep 27(5):873–83

    Article  PubMed  CAS  Google Scholar 

  • U Nagaharu (1935) Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452

    Google Scholar 

  • Walling JG, Jiang JM (2012) DNA and chromatin fiber-based plant cytogenetics. Plant Genet Genomics 4:121–30

    Google Scholar 

  • Wang YP, Sonntag K, Rudloff E, Wehling P, Snowdon RJ (2006) GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines. Plant Cell Rep 25(1):35–40

    Article  PubMed  Google Scholar 

  • Wang XW, Wang HZ, Wang J, Sun RF, Wu J, Liu SY et al (2011) The genome of the mesopolyploid crop species Brassica rapa. Nat Genet 43(10):1035–U157

    Article  PubMed  CAS  Google Scholar 

  • Warwick SI, Al-Shehbaz IA (2006) Brassicaceae: chromosome number index and database on CD-Rom. Plant Syst Evol 259(2–4):237–48

    Article  Google Scholar 

  • Xiong Z, Pires JC (2011) Karyotype and identification of all homoeologous chromosomes of allopolyploid Brassica napus and its diploid progenitors. Genetics 187:37–49

    Article  PubMed  CAS  Google Scholar 

  • Xiong Z, Kim JS, Pires JC (2010) Integration of genetic, physical, and cytogenetic maps for Brassica rapa chromosome A7. Cytogenet Genome Res 129(1–3):190–8

    Article  PubMed  CAS  Google Scholar 

  • Zhang WL, Lee HR, Koo DH, Jiang JM (2008) Epigenetic modification of centromeric chromatin: Hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20(1):25–34

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. School of Agriculture and Food Sciences, The University of Queensland, 4072, Brisbane, Australia

    Annaliese S. Mason Ph.D., B.Sc. (Hons)

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  1. Annaliese S. Mason Ph.D., B.Sc. (Hons)
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Correspondence to Annaliese S. Mason Ph.D., B.Sc. (Hons) .

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Editors and Affiliations

  1. Science and Technology, Division of Plant Breeding and Genetics, S.K. University of Agricultural, Chatha, 180 003, India

    Surinder Kumar Gupta

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Mason, A.S. (2013). Molecular Cytogenetics. In: Gupta, S. (eds) Biotechnology of Crucifers. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7795-2_2

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