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Isolation and Characterization of Prostate Stem Cells

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Abstract

Based on the unique capacity of the rodent prostate to undergo seemingly endless rounds of androgen cycling in response to castration and androgen add-back, the prostate has been proposed to contain long-term self-renewing stem cells. However the prospective isolation and characterization of stem-like cells from rodent and human prostate tissue has only been described over the last 2 decades. Several models of epithelial homeostasis in the adult prostate have been proposed based on either the presence of a multipotent tissue stem cell that differentiates through a series of intermediate developmental stages or the coexistence of multiple unipotent lineage-restricted stem cells. The isolation of cells with stem and progenitor activity is an important first step to delineate the epithelial hierarchy of the prostate. In addition, isolation of stem cells allows characterization of their functional capacities and the molecular programs regulating their activity. These studies will enable detection or targeting of stem and progenitor cells during various stages of neoplastic transformation and tumor progression, including the lethal phase of the disease, castration-resistant prostate cancer.

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Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA

    Andrew S. Goldstein Ph.D. & Owen N. Witte M.D.

  2. Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA

    Andrew S. Goldstein Ph.D.

  3. Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA

    Andrew S. Goldstein Ph.D. & Owen N. Witte M.D.

  4. Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, 90095, USA

    Andrew S. Goldstein Ph.D. & Owen N. Witte M.D.

  5. Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, 90095, USA

    Owen N. Witte M.D.

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  1. Andrew S. Goldstein Ph.D.
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Correspondence to Andrew S. Goldstein Ph.D. .

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  1. Anschutz Medical Campus, University of Colorado, N/A, Aurora, 80045, Colorado, USA

    Scott D. Cramer

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Goldstein, A.S., Witte, O.N. (2013). Isolation and Characterization of Prostate Stem Cells. In: Cramer, S. (eds) Stem Cells and Prostate Cancer. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6498-3_2

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