Abstract
One of the most important questions in microdialysis is how to relate the concentrations in the microdialysate to the true concentrations outside the probe as in vitro recovery was not equal per se to the recovery in vivo. Many calibration techniques and approaches have been proposed, with different levels of practicality and usefulness, to obtain quantitative information on extracellular concentrations as a function of time. Today, we have build up enough experience to know how to obtain quantitative data using the microdialysis technique. With that, it is the only technique that provides data on the unbound concentration of compounds in extracellular fluid spaces in the body, being highly important information in drug development as the unbound concentrations are the drivers for the effect.
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de Lange, E.C.M. (2013). Recovery and Calibration Techniques: Toward Quantitative Microdialysis . In: Müller, M. (eds) Microdialysis in Drug Development. AAPS Advances in the Pharmaceutical Sciences Series, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4815-0_2
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DOI: https://doi.org/10.1007/978-1-4614-4815-0_2
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