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Microdialysis in Drug Development pp 283–302Cite as

Open Flow Microperfusion: An Alternative Method to Microdialysis?

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Part of the book series: AAPS Advances in the Pharmaceutical Sciences Series ((AAPS,volume 4))

Abstract

Membrane-based sampling systems encounter problems when sampling high molecular weight or highly lipophilic substances in the interstitial fluid. Open flow microperfusion (OFM) overcomes these problems by replacing the membrane with a steel mesh featuring macroscopic openings in combination with a peristaltic OFM pump in push/pull mode to achieve stable recovery of OFM samples. Unfiltered sampling results in a complete representation of the ISF for relative and absolute quantification in the target tissue. Current applications in adipose subcutaneous tissue (aOFM) and dermal tissue (dOFM) range from preclinical studies to clinical trials, and cover a wide range of substances from small ions to lipophilic topical drugs to large antibodies. The latest development in OFM has been designed for use in cerebral tissue (cOFM). Currently used in preclinical research, cOFM allows effective sampling in brain tissue with an intact blood–brain barrier. Future work will combine OFM with metabolomics for a more complete assessment of metabolic pathways.

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Authors and Affiliations

  1. Health, Institute for Biomedicine and Health Sciences, Joanneum Research, Graz, Austria

    Thomas Pieber, Thomas Birngruber, Manfred Bodenlenz, Christian Höfferer, Selma Mautner, Katrin Tiffner & Frank Sinner

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  1. Thomas Pieber
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  2. Thomas Birngruber
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  3. Manfred Bodenlenz
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  4. Christian Höfferer
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  5. Selma Mautner
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  6. Katrin Tiffner
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  7. Frank Sinner
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Correspondence to Frank Sinner .

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Editors and Affiliations

  1. Allgemeines Krankenhaus, Department of Clinical Pharmacology, Medical University Vienna, Währinger Gürtel 18-20, Vienna, 1090, Austria

    Markus Müller

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Pieber, T. et al. (2013). Open Flow Microperfusion: An Alternative Method to Microdialysis?. In: Müller, M. (eds) Microdialysis in Drug Development. AAPS Advances in the Pharmaceutical Sciences Series, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4815-0_15

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