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Cytometry-Based Antimicrobial Resistance Techniques

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Abstract

Flow cytometry (FC) has been regarded as one of the most promising technologies for the clinical microbiology laboratories for nearly three decades. The accuracy, versatility, and quantitative nature of the FC measurements are both complementary and superior to many microscopic and other techniques used in the clinical microbiology laboratories. Since early 1990s, numerous proof-of-concept studies have appeared in the literature for the FC identification of pathogenic bacteria, fungi, parasites, and viruses, and for the rapid testing of susceptibility of these pathogens to antimicrobials and other compounds. The reader is referred to a number of comprehensive review articles that describe the principles of FC, reagents, protocols, and a variety of applications for specific pathogens [1–6]. There are also excellent studies describing the use of FC and bead arrays, using antigen, antibody, or nucleic acid probes, for the identification and genotyping of microbes [7–9].

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Author information

Authors and Affiliations

  1. Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY, 12208, USA

    Vishnu Chaturvedi Ph.D.

  2. Department of Biomedical Sciences, University at Albany School of Public Health, Albany, NY, USA

    Vishnu Chaturvedi Ph.D.

Authors
  1. Vishnu Chaturvedi Ph.D.
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Correspondence to Vishnu Chaturvedi Ph.D. .

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Editors and Affiliations

  1. Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, 10065, New York, USA

    Yi-Wei Tang

  2. Vanderbilt Clinic, Clinical Microbiology Laboratory, Vanderbilt University Medical Center, 22nd Avenue 1301, Nashville, 37232-5310, Tennessee, USA

    Charles W. Stratton

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Chaturvedi, V. (2013). Cytometry-Based Antimicrobial Resistance Techniques. In: Tang, YW., Stratton, C. (eds) Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-3970-7_5

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