Abstract
Drug resistance testing is recommended for the management of patients with HIV-1 infection. Celera ViroSeq™ HIV-1 Genotyping System is the most widely used genotypic HIV drug resistance testing. The assay produces 1,302 nucleotide sequences comprising codons 1–99 of the protease gene and 1–335 of the reverse transcriptase gene. Genotyping result is linked to an interpretive algorithm, for example, Virco® TYPE HIV-1 to generate drug resistance profile.
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We would like to thank Dr. Natalia M. Marlowe for critical review and comments of the chapter.
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Disclaimer: The contents of this chapter represent modified ViroSeq protocol which has been validated at the Department of Pathology, University of Texas Medical Branch
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Xu, F., Dong, J. (2012). HIV-1 Genotyping and Virtual Phenotyping. In: Hu, P., Hegde, M., Lennon, P. (eds) Modern Clinical Molecular Techniques. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2170-2_8
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DOI: https://doi.org/10.1007/978-1-4614-2170-2_8
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