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Oxygen Transport to Tissue XXXIII pp 119–124Cite as

Use of a Hybrid Optical Spectrometer for the Measurement of Changes in Oxidized Cytochrome c Oxidase Concentration and Tissue Scattering During Functional Activation

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 737))

Abstract

We report the use of a novel dual-channel hybrid near-infrared optical spectrometer for the measurement of changes in oxidized cytochrome c oxidase (oxCCO) concentration and optical scattering in 11 healthy volunteers during functional activation induced by an anagram-solving task. A hemodynamic Changed ‘haemodynamic’ to ‘hemodynamic’ though followed UK spelling. Please check. response consistent with functional activation was seen in 9 out of 11 subjects and in 13 of the total 22 channels measured; only these channels were retained for further analysis. In each of these 13 channels the hemodynamic response was accompanied by a statistically significant change in [oxCCO], although the direction of this change was heterogenous with a significant increase seen in eight channels and a significant decrease seen in five channels. There was no significant change in the optical scattering coefficient measured at four wavelengths, and the use of differential pathlength factor (DPF) calculated in real-time using measured absorption and scattering showed a high level of agreement with a conventional algorithm. The possible reasons for the heterogeneity of the [oxCCO] response are discussed.

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Acknowledgements

This work was undertaken at University College London Hospitals and partially funded by the Department of Health’s National Institute for Health Research Centres funding scheme. This research was supported by MRC program grant G0701458 and EPSRC grant EP/D0609821/1. IT is supported by Wellcome Trust grant 088429/Z/09/Z.

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Authors and Affiliations

  1. Neurosurgical Critical Care Unit, National Hospital for Neurology and Neurosurgery, University College London Hospitals, London, UK

    Arnab Ghosh & Martin Smith

  2. Biomedical Optics Research Laboratory, Department of Medical Physics and Bioengineering, University College London, Malet Place Engineering Building, London, WC1E 6BT, UK

    Ilias Tachtsidis, Christina Kolyva, Martin Smith & Clare E. Elwell

  3. Department of Biological Sciences, University of Essex, Colchester, UK

    Chris E. Cooper

Authors
  1. Arnab Ghosh
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  2. Ilias Tachtsidis
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  3. Christina Kolyva
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  4. Chris E. Cooper
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  5. Martin Smith
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  6. Clare E. Elwell
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Corresponding author

Correspondence to Clare E. Elwell .

Editor information

Editors and Affiliations

  1. , Division of Neonatology, University Hospital Zurich, Frauenklinikstr. 10, Zurich, 8091, Switzerland

    Martin Wolf

  2. , Division of Neonatology, University Hospital Zurich, Frauenklinikstr. 10, Zurich, 8091, Switzerland

    Hans Ulrich Bucher

  3. Institute for Biomedical Engineering, University of Zurich/ETHZ, Wolfgang-Pauli-Strasse 27, Zurich, 8093, Switzerland

    Markus Rudin

  4. ESAT-SCD(SISTA), Dept. Elektrotechniek, Katholieke Universiteit Leuven, Kasteelpark Arenberg 10, Leuven-Heverlee, 3001, Belgium

    Sabine Van Huffel

  5. , Institute of Complementary Medicine KIKO, University of Bern, Imhoof-Pavillon, Inselspital, Bern, 3010, Switzerland

    Ursula Wolf

  6. Synthesizer Inc., Synthesizer Inc. 2773, Ellicott City, 21043, Maryland, USA

    Duane F. Bruley

  7. , Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom

    David K Harrison

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Ghosh, A., Tachtsidis, I., Kolyva, C., Cooper, C.E., Smith, M., Elwell, C.E. (2012). Use of a Hybrid Optical Spectrometer for the Measurement of Changes in Oxidized Cytochrome c Oxidase Concentration and Tissue Scattering During Functional Activation. In: Wolf, M., et al. Oxygen Transport to Tissue XXXIII. Advances in Experimental Medicine and Biology, vol 737. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-1566-4_18

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