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Nonviral Gene Delivery for Applications in Regenerative Medicine

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Book cover Engineering Biomaterials for Regenerative Medicine

Abstract

To promote tissue repair and regeneration, much research in the field of tissue engineering has been aimed at the development of synthetic three-dimensional scaffolds to maintain the space and provide the mechanical support necessary for tissue development. However, to regenerate functional tissue of the same quality as natural tissue, the release of biochemical cues from these synthetic matrices will be necessary. While both bolus injection as well as polymeric encapsulation of proteins has been shown to stimulate regenerative processes, proteins have a fragile three-dimensional structure, which can be costly and difficult to synthesize. Because of the increased stability of DNA in comparison with proteins, plasmids may be used to stimulate gene transfer and localized expression of plasmid-encoded proteins to promote tissue development. However, due to the multiple barriers to gene transfer, a gene delivery vehicle must be carefully designed to impart control over the spatial and temporal release of the DNA. Furthermore, as the cellular processes involved in directing tissue repair are complex, delivery must be well controlled and stimulate gene expression that mimics the natural release processes of target growth factors and other proteins. Thus, this chapter will discuss the delivery control imparted by various mechanisms of gene transfer including bolus, polymeric, and substrate-mediated delivery. Matrix-controlled delivery methods for regenerative medicine applications will be explored in depth. Ultimately, to reform tissue of the necessary quality and functionality, the appropriate spatial and temporal patterning of gene expression profiles within targeted cells will need to be attained by synthetic systems.

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    Kory Blocker & Millicent Sullivan

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    Sujata K. Bhatia

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Blocker, K., Sullivan, M. (2012). Nonviral Gene Delivery for Applications in Regenerative Medicine. In: Bhatia, S. (eds) Engineering Biomaterials for Regenerative Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-1080-5_11

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