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Assembly and Architecture of HIV

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Viral Molecular Machines

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 726))

Abstract

HIV forms spherical, membrane-enveloped, pleomorphic virions, 1,000–1,500 Å in diameter, which contain two copies of its single-stranded, positive-sense RNA genome. Virus particles initially bud from host cells in a noninfectious or immature form, in which the genome is further encapsulated inside a spherical protein shell composed of around 2,500 copies of the virally encoded Gag polyprotein. The Gag molecules are radially arranged, adherent to the inner leaflet of the viral membrane, and closely associated as a hexagonal, paracrystalline lattice. Gag comprises three major structural domains called MA, CA, and NC. For immature virions to become infectious, they must undergo a maturation process that is initiated by proteolytic processing of Gag by the viral protease. The new Gag-derived proteins undergo dramatic rearrangements to form the mature virus. The mature MA protein forms a “matrix” layer and remains attached to the viral envelope, NC condenses with the genome, and approximately 1,500 copies of CA assemble into a new cone-shaped protein shell, called the mature capsid, which surrounds the genomic ribonucleoprotein complex. The HIV capsid conforms to the mathematical principles of a fullerene shell, in which the CA subunits form about 250 CA hexamers arrayed on a variably curved hexagonal lattice, which is closed by incorporation of exactly 12 pentamers, seven pentamers at the wide end and five at the narrow end of the cone. This chapter describes our current understanding of HIV’s virion architecture and its dynamic transformations: the process of virion assembly as orchestrated by Gag, the architecture of the immature virion, the virus maturation process, and the structure of the mature capsid.

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Abbreviations

HIV-1:

Human immunodeficiency virus type 1

HTLV:

Human T-cell leukemia virus

MLV:

Murine leukemia virus

MPMV:

Mason-Pfizer monkey virus

RSV:

Rous sarcoma virus

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Acknowledgments

HIV research in the Yeager laboratory is supported by grants from the US National Institutes of Health (RO1-GM066087 and P50-GM082545). We thank John Briggs, Rebecca Craven, Alasdair Steven, and Elizabeth Wright for generously supplying materials for figures; Kelly Dryden and Jeong-Hyun Lee for assistance in preparing figures; and Wes Sundquist for helpful discussions. We apologize to colleagues whose works were not cited due to lack of space or inadvertent omission.

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  1. Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, 800736, Charlottesville, VA, 22908, USA

    Barbie K. Ganser-Pornillos, Mark Yeager & Owen Pornillos

  2. Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Barbie K. Ganser-Pornillos, Mark Yeager & Owen Pornillos

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  1. Barbie K. Ganser-Pornillos
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  2. Mark Yeager
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  3. Owen Pornillos
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Correspondence to Barbie K. Ganser-Pornillos , Mark Yeager or Owen Pornillos .

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  1. Dept. Biological Sciences, Purdue University, W. State St. 915, West Lafayette, 47907-2054, Indiana, USA

    Michael G. Rossmann

  2. Dept. Biology, Catholic University of America, Washington, 20064, District of Columbia, USA

    Venigalla B. Rao

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Ganser-Pornillos, B.K., Yeager, M., Pornillos, O. (2012). Assembly and Architecture of HIV. In: Rossmann, M., Rao, V. (eds) Viral Molecular Machines. Advances in Experimental Medicine and Biology, vol 726. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-0980-9_20

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