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UBF an Essential Player in Maintenance of Active NORs and Nucleolar Formation

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The Nucleolus

Part of the book series: Protein Reviews ((PRON,volume 15))

Abstract

Nucleolar organizer regions (NORs) are comprised of tandem arrays of ribosomal gene repeats. During interphase, ribosomal genes are transcribed by RNA polymerase I resulting in the formation of a nucleolus. Within nucleoli, an intricate and highly coordinated assembly pathway is responsible for the production of biology’s most complex machine, the ribosome. Upstream binding factor (UBF) binds extensively and plays a key role in organizing ribosomal gene chromatin throughout the cell cycle. It is responsible for the appearance of active NORs as secondary constrictions on metaphase chromosomes and its levels determine the proportion of ribosomal gene repeats that are active in a given cell type. Extensive UBF binding to NORs directs recruitment of many factors required in the early steps of ribosome biogenesis, thus enabling efficient nucleolar reformation. Finally, we reveal that UBF, once thought to be restricted to vertebrates, is present in many animal phyla.

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Acknowledgments

We would like to thank Tom Moss and Olivier Gadal for communicating results prior to publication. Work in the McStay laboratory is funded by PI grant number 07/IN.1/B924 from Science Foundation Ireland. Alice Grob is funded by a postdoctoral fellowship from IRCSET.

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Authors and Affiliations

  1. Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Alice Grob, Christine Colleran & Brian McStay

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  1. Alice Grob
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  2. Christine Colleran
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  3. Brian McStay
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Correspondence to Brian McStay .

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  1. Dept. Biochemistry, University of Mississippi Medical Center, North State St. 2500, Jackson, 39216, Mississippi, USA

    Mark O. J. Olson

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Grob, A., Colleran, C., McStay, B. (2011). UBF an Essential Player in Maintenance of Active NORs and Nucleolar Formation. In: Olson, M. (eds) The Nucleolus. Protein Reviews, vol 15. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0514-6_5

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