Abstract
Since its introduction (Ohnishi and McConnell, 1965; Stone et al.9 1965), the spin-labeling technique has enjoyed great success in applications involving quantitation of molecular motion in biological systems. Early studies were largely devoted to characterizations of the nanosecond rotational motions of small soluble proteins and lipid components in membrane bilayers using linear1electron paramagnetic resonance (EPR) spectroscopy. However, development of continuous wave saturation transfer electron paramagnetic resonance (cw-ST-EPR) spectroscopy (Hyde and Dalton, 1972; Hyde and Thomas, 1973; Thomas et al., 1976) and time domain techniques including saturation recovery EPR (SR-EPR; Huisjen and Hyde, 1974; Hyde, 1979; Freed, 1979; Fajer et al., 1986) has provided access to a new motional window which is important for studies of dynamics and interactions of membrane proteins and interactions of soluble proteins to form supramolecular structures. While linear EPR spectroscopy provides sensitivity to rotational motions in the 10 ps to 1 µs correlation time range using nitroxide spin labels (McCalley et al, 1972), cw-ST-EPR provides high sensitivity in the 1µs to 1 ms correlation time range (Thomas et al, 1976) and SR-EPR at least in the 1 to 15 µs range (Fajer et al, 1986). The range of rotational times spanned by cw-ST-EPR, generally referred to as the very slow motional region, is characteristic of proteins embedded in lipid bilayers and of single proteins or protein-protein complexes of combined molecular weight≥300 kDa in aqueous media.
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References
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Beth, A.H., Robinson, B.H. (1989). Nitrogen-15 and Deuterium Substituted Spin Labels for Studies of Very Slow Rotational Motion. In: Berliner, L.J., Reuben, J. (eds) Spin Labeling. Biological Magnetic Resonance, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0743-3_4
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