The Sister Chromatid Exchange Test
- Sheldon Wolff
- … show all 1 hide
Purchase on Springer.com
$29.95 / €24.95 / £19.95*
* Final gross prices may vary according to local VAT.
Mutagenic carcinogens such as alkylating agents, or physical mutagens such as ultra-violet light, induce lesions in DNA. When DNA containing such lesions replicates during S-phase, exchanges often occur between the two daughter molecules (Wolff et al, 1974). At the cytological level this becomes manifest as exchanges between the two sister chromatids of a metaphase chromosome. To visualize these exchanges, however, the cells must be treated so that the two sister chromatids will be different from one another. This can be accomplished by exploiting the fact that DNA is a double molecule that replicates semiconservatively, i.e., it has two complementary polynucleotide strands that separate from one another, and each acts as a template for the synthesis of new complementary strands. This means that each newly synthesized DNA molecule consists of one old polynucleotide strand that is conserved and a brand new strand.
- Allen, J.W., Latt, S.A. (1976): In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells. Chromosoma 58: 325–340. CrossRef
- Allen, J.W., Shuler, C.F., Mendes, R.W., Latt, S.A. (1977): A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromodeoxyuridine tablets. Cytogenet. Cell Genet. 18: 231–237. CrossRef
- Ames, B.N., McCann, J., Yamasaki, E. (1975): Methods for testing carcinogens and mutagens with the Salmonella/mammalian- microsome mutagenicity test. Mutat. Res. 31: 347–364.
- Bloom, S.E., Hsu, T.C. (1975): Differential fluorescence of sister chromatids in chicken embryos exposed to 5-bromodeoxyuridine. Chromosoma 51: 261–267. CrossRef
- Gatti, M., Santini, G., Pimpinelli, S., Olivieri, G. (1979): Lack of spontaneous sister chromatid exchanges in somatic cells of Drosophila melanogaster. Genetics 91: 255–274.
- Goto, K., Akematsu, T., Shimazu, H., Sugiyama, T. (1975): Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining. Chromosoma 53: 223–230. CrossRef
- Ikushima, T., Wolff, S. (1974): Sister chromatid exchanges induced by light flashes to 5- bromodeoxyuridine and 5-iododeoxyuridine substituted Chinese hamster chromosomes. Exp. Cell Res. 87: 15–19. CrossRef
- Kato, H. (1974): Spontaneous sister chromatid exchanges detected by a BUdR-labelling method. Nature (Lond.) 251: 70–72. CrossRef
- Kato, H., Shimada, H. (1975): Sister chromatid exchanges induced by mitomycin C: a new method of detecting DNA damage at chromosomal level. Mutat. Res. 28: 459–464. CrossRef
- Kihlman, B.A., Kronborg, D. (1975): Sister chromatid exchanges in Viciafaba. I. Demonstration by a modified fluorescent plus Giemsa (FPG) technique. Chromosoma 51: 1–10. CrossRef
- Kim, M.A. (1974): Chromatidaustauschund He- terochromatinveranderungen menschlicher Chromosomen nach BUdR-Markierung. Humangenetik 25: 179–188. CrossRef
- Kligerman, A.D., Bloom, S.E. (1976): Sister chromatid differentiation and exchanges in adult mudminnows (Umbra limi) after in vivo exposure to 5-bromodeoxyuridine. Chromosoma 56: 101–109. CrossRef
- Korenberg, J., Freedlender, E.F. (1974): Giemsa technique for the detection of sister chromatid exchanges. Chromosoma 48: 355–360. CrossRef
- Latt, S.A. (1973): Microfluorometry detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. Natl. Acad. Sci. USA 70: 3395–3399. CrossRef
- Latt, S.A. (1974): Sister chromatid exchanges, indices of human chromosome damage and repair: Detection by fluorescence and induction by mitomycin C. Proc. Natl. Acad. Sci. USA 71: 3162–3166. CrossRef
- Lin, M.S., Alfi, O.S. (1976): Detection of sister chromatid exchanges by 4’-6-diamidino-2- phenylindole fluorescence. Chromosoma 57: 219–225. CrossRef
- Mailing, H.V. (1971): Dimethylnitrosamine: formation of mutagenic compounds by interaction with mouse liver microsomes. Mutat. Res. 13: 425–429. CrossRef
- Natarajan, A.T., Tates, A.D., van Buul, P.P.W., Meijers, M., de Vogel, N. (1976): Cytogenetic effects of mutagens/carcinogens after activation in a microsomal system in vitro. I. Induction of chromosome aberrations and sister chromatid exchanges by diethylnitrosamine (DEN) and dimethylnitrosamine (DMN) in CHO cells in the presence of rat-liver microsomes. Mutat. Res. 37: 83–90. CrossRef
- Perry, P., Evans, H.J. (1975): Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature (Lond.) 258: 121–125. CrossRef
- Perry, P., Wolff, S. (1974): New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251: 156–158. CrossRef
- Schneider, E.L., Chaillet, J.R., Tice, R.R. (1976): In vivo BUdR labeling of mammalian chromosomes. Exp. Cell Res. 100: 396–399. CrossRef
- Solomon, E., Bobrow, M. (1975): Sister chromatid exchanges—a sensitive assay of agents damaging human chromosomes. Mutat. Res. 30: 273–278.
- Stetka, D.G., Wolff, S. (1976a): Sister chromatid exchange as an assay for genetic damage induced by mutagen-carcinogens. I. In vivo test for compounds requiring metabolic activation. Mutat. Res. 41: 333–342.
- Stetka, D.G., Wolff, S. (1976b): Sister chromatid exchange as an assay for genetic damage induced by mutagen-carcinogens. II. In vitro test for compounds requiring metabolic activation. Mutat. Res. 41: 343–350.
- Takehisa, S., Wolff, S. (1977): Induction of sister chromatid exchanges in Chinese hamster cells by carcinogenic mutagens requiring metabolic activation. Mutat. Res. 45: 263–270. CrossRef
- Takehisa, S., Wolff, S. (1978): The induction of sister-chromatid exchanges in Chinese hamster ovary cells by prolonged exposure to 2- acetylaminofluorene and S-9 Mix. Mutat. Res. 58: 103–106. CrossRef
- Taylor, J.H., Woods, P.S., Hughes, W.L. (1957): The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labeled thymidine. Proc. Natl. Acad. Sci. USA 43: 122–128. CrossRef
- Terasima, T., Tolmach, L.J. (1961): Changes in X-ray sensitivity of HeLa cells during the division cycle. Nature (Lond.) 190: 1210–1211. CrossRef
- Tice, R., Chaillet, J., Schneider, E.L. (1976): Demonstration of spontaneous sister chromatid exchanges in vivo. Exp. Cell Res. 102: 426–429. CrossRef
- Wienberg, J. (1977): BrdU-Giemsa-technique for the differentiation of sister chromatids in somatic cells of Drosophila melanogaster. Mutat. Res. 44: 283–286. CrossRef
- Wolff, S. (1980): Sister chromatid exchange in mammalian cells. In: Handbook of DNA Repair Techniques, Friedberg, E.C., and Hanawalt, P.C. (eds.), Vol. 5, New York: Marcel Dekker, Inc.
- Wolff, S., Perry, P. (1974): Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma 48: 341–353. CrossRef
- Wolff, S., Rodin, B. (1978): Saccharin-induced sister chromatid exchanges in Chinese hamster and human cells. Science 200: 543–545. CrossRef
- Wolff, S., Bodycote, J., Painter, R.B. (1974): Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: The necessity for cells to pass through S. Mutat. Res. 25: 73–81. CrossRef
- Zakharov, A.F., Egolina, N.A. (1972): Differential spiralization along mammalian mitotic chromosomes. I. BUdR-revealed differentiation in Chinese hamster chromosomes. Chromosoma 38: 341–365. CrossRef
- The Sister Chromatid Exchange Test
- Book Title
- Short-Term Tests for Chemical Carcinogens
- Book Part
- pp 236-242
- Print ISBN
- Online ISBN
- Series Title
- Topics in Environmental Physiology and Medicine
- Series ISSN
- Springer New York
- Copyright Holder
- Springer-Verlag New York
- Additional Links
- Industry Sectors
- eBook Packages
To view the rest of this content please follow the download PDF link above.