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Selected Topics in Micro/Nano-robotics for Biomedical Applications pp 125–158Cite as

Investigation of Protein–Protein Interactions in Cancer Targeted Therapy Using Nanorobots

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Abstract

This chapter describes the applications of nanorobots in investigating the mechanisms of rituximab’s different efficacies in the targeted therapy of B-cell lymphomas at the individual cellular/molecular level. The chapter begins with an introduction to the new challenges in the field of cancer targeted therapy, taking rituximab targeted therapy in B-cell lymphoma for example. The following section presents a detailed description of the principles and methods of single-molecule techniques based on nanorobots. Next, it presents the microfabricated pillar-based cell immobilization method and discusses how to obtain the topography of individual living mammalian suspension cells based on this immobilization method. Next, it presents how to use nanorobot indentation experiments to measure the mechanical properties of individual cells. Next, the processes of using nanorobots to measure the individual molecular binding forces and three-dimensionally visualize the distribution of CD20 proteins on the lymphoma cell surface with the probe functionalization technology are detailed. The remainder of the chapter presents the specific binding force measurements on different lymphoma patients’ cells and discusses its relation to rituximab’s variable efficacies. The intent of this chapter is to provide the practical knowledge to begin the investigations on individual cells and molecules with nanorobots.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Project No.60904095, 61175103), and the CAS FEA International Partnership Program for Creative Research Teams.

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Authors and Affiliations

  1. State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China

    Mi Li, Lianqing Liu, Yuechao Wang & Zaili Dong

  2. Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA

    Ning Xi

  3. Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China

    Xiubin Xiao & Weijing Zhang

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  1. Mi Li
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  2. Lianqing Liu
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  3. Ning Xi
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  4. Yuechao Wang
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  5. Zaili Dong
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  6. Xiubin Xiao
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  7. Weijing Zhang
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Correspondence to Lianqing Liu or Ning Xi .

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  1. , Department of Electrical and, Stevens Institute of Technology, Hoboken, 07030, New Jersey, USA

    Yi Guo

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Li, M. et al. (2013). Investigation of Protein–Protein Interactions in Cancer Targeted Therapy Using Nanorobots. In: Guo, Y. (eds) Selected Topics in Micro/Nano-robotics for Biomedical Applications. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-8411-1_8

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  • DOI: https://doi.org/10.1007/978-1-4419-8411-1_8

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