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Insertional Mutagenesis Strategies in Cancer Genetics pp 39–75Cite as

Gene Discovery by MMTV Mediated Insertional Mutagenesis

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Abstract

Transformation of a normal cell into a cancer cell requires sequential accumulation of several genetic changes that affect various collaborating signaling cascades mainly involved in cell proliferation, survival and development [46]. To develop novel specific therapeutic compounds for cancer treatment, identification of these cancer causing genes, and subsequently the oncogenic pathways in which these genes act, is of utmost importance. Although a great deal of insight in the cellular mechanisms that lead to cancer has been obtained, many key players are still unidentified. Retroviral insertional mutagenesis (IM) screens provide one of the most efficient tools to identify genes involved in tumorigenesis and from there the specific oncogenic pathways involved.

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    Annabel Vendel-Zwaagstra & John Hilkens

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    Adam J. Dupuy

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    David A. Largaespada

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Vendel-Zwaagstra, A., Hilkens, J. (2011). Gene Discovery by MMTV Mediated Insertional Mutagenesis. In: Dupuy, A., Largaespada, D. (eds) Insertional Mutagenesis Strategies in Cancer Genetics. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7656-7_3

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