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Imaging Protein Interactions in Living Cells Using the Fluorescent Proteins

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Reviews in Fluorescence 2008

Part of the book series: Reviews in Fluorescence 2008 ((RFLU,volume 2008))

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Abstract

Over the past decade, the visible fluorescent proteins (VFPs) have become an essential tool for the study of cell biology and physiology. The goal of this chapter is to describe the characteristics of the FPs and discuss the limits of their use as probes for noninvasive imaging of protein behavior in living cells. We also described live-cell imaging techniques used to monitor the subcellular dynamics and interactions of proteins in living cells. We then focused on the method of fluorescence resonance energy transfer (FRET) microscopy to quantify the interactions of proteins inside living cells and consider some of the factors that limit this approach.

This chapter is dedicated to our colleagues at Tulane University who lost so much in the aftermath of hurricane Katrina, August 29, 2005.

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Authors and Affiliations

  1. Departments of Medicine and Cell Biology, University of Virginia Health Services, Charlottesville, VA, 22908, USA

    Richard N. Day

  2. Keck Center for Cellular Imaging, Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA, 22904, USA

    Ammasi Periasamy

  3. Department of Cell Biology, University of Virginia Health Services, Charlottesville, VA, 22908, USA

    Ignacio Demarco

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  1. Richard N. Day
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  2. Ammasi Periasamy
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  3. Ignacio Demarco
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Correspondence to Ammasi Periasamy .

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  1. Institute of Fluorescence, University of Maryland, 701 E. Pratt St., Suites 3017-21, Baltimore, MD, 21202, USA

    Chris D. Geddes

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Day, R.N., Periasamy, A., Demarco, I. (2010). Imaging Protein Interactions in Living Cells Using the Fluorescent Proteins. In: Geddes, C.D. (eds) Reviews in Fluorescence 2008. Reviews in Fluorescence 2008, vol 2008. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1260-2_14

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