Variables Affecting the Cutting Properties of Tissues and the Resulting Artifacts

Abstract

This chapter elucidates the variables effecting the way tissues cut when preparing frozen sections. Our goal is to recognize artifacts on the sections as they are being cut and make corrections and adjustments to yield the best sections possible. The cutting properties of tissue with varying temperature are discussed in detail with illustrations of artifacts and techniques for adjusting block temperature and cutting ribbons. The cutting properties of various types of tissues are described with suggestions for dealing with difficult-to-section tissues such as fatty tissues and tough or hard tissues. Other parameters, such as the amount of tissue and thickness of the sections being cut, are discussed. Suggestions for dealing with common problems such as sectioning fatty specimens and cutting tissues that are curling away, common artifacts and suggested remedies, are described.