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Ribonuclease P pp 113–134Cite as

Roles of Metal Ions in RNase P Catalysis

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Part of the book series: Protein Reviews ((PRON,volume 10))

Abstract

As for other RNA, RNase P with its catalytic RNA subunit requires metal(II)-ions for function. Approximately 100 metal(II)-ions bind to the 400 residues long RNA and several to the precursor substrate, e.g., roughly 25–30 to a tRNA precursor substrate. To understand the function and the reaction catalyzed by RNase P an important task is to identify and characterize metal(II)-ions or metal binding sites that contribute to folding of the RNAs, interaction with the protein subunit(s), substrate binding and chemistry of cleavage. Over the years, different methods have been explored to extract information about how, were and when metal(II)-ions bind to RNA. In this chapter, I will discuss our current understanding of RNase P and metal(II)-ions and how this knowledge can be utilized to search for new candidate drugs referred to as metal mimics.

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Acknowledgments

I thank my colleagues over the years for a pleasant and stimulating working atmosphere, Dr. S. Dasgupta for comments on the manuscript, and Drs N.E. Mikkelsen and S. Trobro for help with the figures 1, 2 and 3. This work was supported by the Strategic Research Foundation, and the Swedish Research Council.

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  1. Department of Cell and Molecular Biology, Biomedical Center, Uppsala University, 596, Uppsala, Sweden

    Leif A. Kirsebom

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Correspondence to Leif A. Kirsebom .

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  1. School of Public Health, University of California, Berkeley, Warren Hall 140, Berkeley, 94720, U.S.A.

    Fenyong Liu

  2. Dept. Molecular, Cellular &, Yale University, Whitney Avenue 266, New Haven, 06520-8104, U.S.A.

    Sidney Altman

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Kirsebom, L.A. (2010). Roles of Metal Ions in RNase P Catalysis. In: Liu, F., Altman, S. (eds) Ribonuclease P. Protein Reviews, vol 10. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1142-1_7

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