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Novel Methodologies: Proteomic Approaches in Substance Abuse Research

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Addiction Medicine

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Abstract

Proteomics is the study of sequence, expression, function, interaction and localization of proteins in tissues or fluids of various organisms. With the advent of proteomic techniques providing greater selectivity, sensitivity and throughput, drug abuse researchers are faced with decisions as to the selection of the most appropriate applications for their respective questions. In addition, drug abuse researchers face additional challenges that are unique to neuroscience limited sample quantities, heterogeneous cellular compositions of samples and the hydrophobic nature of large classes of proteins of interest. The purpose of this chapter is provide the drug abuse researcher with current technologies and methodologies for examining coordinated regulation of multiple proteins in tissue, primarily brain. Chapter content includes useful information on subcellular organelle isolation, protein fractionation and separation using two-dimensional gel electrophoresis and multi-dimensional liquid chromatography, methods for quantification of differential protein expression (e.g., two-dimensional difference in gel electrophoresis and isotope-coded affinity tags (ICAT and iTRAQ)), and mass spectrometry approaches. An overview of the techniques used currently to assign post-translational modification status on a proteomics scale is also evaluated. The application of these approaches to the study of cocaine and alcohol abuse is reviewed.

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Acknowledgements

Supported in part by funding of the following NIH grants: DA012498, DA003628, DA06634 (SEH).

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  1. Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USA

    Scott E. Hemby & Nilesh S. Tannu

  2. Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, VA, USA

    Wendy J. Lynch

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Correspondence to Scott E. Hemby .

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  1. , Depts Psychiat & Neurobehav Sciences, Me, University of Virginia, Charlottesville, 22908, Virginia, USA

    Bankole A. Johnson

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Hemby, S.E., Lynch, W.J., Tannu, N.S. (2010). Novel Methodologies: Proteomic Approaches in Substance Abuse Research. In: Johnson, B. (eds) Addiction Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-0338-9_16

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