Abstract
Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various plant growth, development and defense against pathogens. Plant NADPH oxidases are called regulatory burst oxidase homolog or Rboh. Although Rboh has been isolated from numerous plant species, the molecular mechanism of the regulation of its enzymatic activity remains unclear. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+-binding EF-hand motifs. This N-terminal region may provide an entry point for Ca2+ to participate in ROS signaling. In phagocyte NADPH oxidase, the small GTPase Rac2 plays the role of a key regulator. In plants, Rac GTPases are also involved in regulation of ROS production and resistance to pathogens in rice. This review discusses recent findings that provide key clues to the understanding of molecular regulation of Rboh by Rac GTPase.
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References
Apel, K., and Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55, 373–399.
Babior, B.M. (2004). NADPH oxidase. Curr. Opin. Immunol. 16, 42–47.
Baxter-Burrell, A., Yang, Z., Springer, P.S., and Bailey-Serres, J. (2002). RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance. Science 296,2026–2028.
Bindschedler, L.V., Minibayeva, F., Gardner, S.L., Gerrish, C., Davies, D.R., and Bolwell, G.P. (2001). Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2+. New Phytol. 151, 185–194.
Blume, B., Nurnberger, T., Nass, N., and Scheel, D. (2000). Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley. Plant Cell 12, 1425–1440.
Foreman, J., Demidchik, V., Bothwell, J.H., Mylona, P., Miedema, H., Torres, M.A., Linstead, P., Costa, S., Brownlee, C., Jones, J.D., Davies, J.M., and Dolan, L. (2003). Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature 422, 442–446.
Gapper, C., and Dolan, L. (2006). Control of plant development by reactive oxygen species. Plant Physiol. 141, 341–345.
Geiszt, M., and Leto, T.L. (2004). The Nox family of NAD(P)H oxidases: host defense and beyond. J. Biol. Chem. 279, 51715–51718.
Grant, J.J., and Loake, G.J. (2000). Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiol. 124, 21–29.
Jabs, T., Tschope, M., Colling, C., Hahlbrock, K., and Scheel, D. (1997). Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley. Proc. Natl. Acad. Sci. USA 94, 4800–4805.
Kawasaki, T., Henmi, K., Ono, E., Hatakeyama, S., Iwano, M., Satoh, H., and Shimamoto, K. (1999). The small GTP-binding protein rac is a regulator of cell death in plants. Proc. Natl. Acad. Sci. USA 96, 10922–10926
Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N., and Yoshioka, H. (2007). Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase. Plant Cell 19, 1065–1080.
Kurusu, T., Yagala, T., Miyao, A., Hirochika, H., and Kuchitsu, K. (2005). Identification of a putative voltage-gated Ca2+ channel as a key regulator of elicitor-induced hypersensitive cell death and mitogen-activated protein kinase activation in rice. Plant J. 42, 798–809.
Kwak, J.M., Mori, I.C., Pei, Z.M., Leonhardt, N., Torres, M.A., Dangl, J.L., Bloom, R.E., Bodde, S., Jones, J.D., and Schroeder, J.I. (2003). NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis. EMBO J. 22, 2623–2633.
Lambeth, J.D. (2004). NOX enzymes and the biology of reactive oxygen. Nat. Rev. Immunol. 4, 181–189.
Morel, J., Fromentin, J., Blein, J.P., Simon-Plas, F., and Elmayan, T. (2004). Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell. Plant J. 37, 282–293.
Ono, E., Wong, H.L., Kawasaki, T., Hasegawa, M., Kodama, O., and Shimamoto, K. (2001). Essential role of the small GTPase Rac in disease resistance of rice. Proc. Natl. Acad. Sci. USA 98, 759–764.
Park, J., Gu, Y., Lee, Y., and Yang, Z. (2004). Phosphatidic acid induces leaf cell death in Arabidopsis by activating the Rho-related small G protein GTPase-mediated pathway of reactive oxygen species generation. Plant Physiol. 134, 129–136.
Pei, Z.M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G.J., Grill, E., and Schroeder, J.I. (2000). Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406, 731–734.
Sagi, M., and Fluhr, R. (2001). Superoxide production by plant homologues of the gp91(phox) NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection. Plant Physiol. 126, 1281–1290.
Suharsono, U., Fujisawa, Y., Kawasaki, T., Iwasaki, Y., Satoh, H., and Shimamoto, K. (2002). The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice. Proc. Natl. Acad. Sci. USA 99, 13307–13312.
Torres, M.A., and Dangl, J.L. (2005). Functions of the respiratory burst oxidase in biotic interactions, abiotic stress and development. Curr. Opin. Plant Biol. 8, 397–403.
Torres, M.A., Dangl, J.L., and Jones, J.D. (2002). Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc. Natl. Acad. Sci. USA 99, 517–522.
Torres, M.A., Jones, J.D., and Dangl, J.L. (2006). Reactive oxygen species signaling in response to pathogens. Plant Physiol. 141, 373–378.
Ward, J.M., and Schroeder, J.I. (1994). Calcium-Activated K+ Channels and Calcium-Induced Calcium Release by Slow Vacuolar Ion Channels in Guard Cell Vacuoles Implicated in the Control of Stomatal Closure. Plant Cell 6, 669–683.
Wong, H.L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T., and Shimamoto, K. (2007). Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. Plant Cell 19, 4022–4034.
Yang, Z., and Fu, Y. (2007). ROP/RAC GTPase signaling. Curr. Opin. Plant Biol. 10, 490–494.
Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D., and Doke, N. (2003). Nicotiana benthamiana gp91phox homologs NbrbohA and NbrbohB participate in H2O2 accumulation and resistance to Phytophthora infestans. Plant Cell 15, 706–718.
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Wong, H.L., Kawasaki, T., Shimamoto, K. (2009). Rac GTPase and the Regulation of NADPH Oxidase in Rice Innate Immunity Response. In: Wang, GL., Valent, B. (eds) Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9500-9_17
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DOI: https://doi.org/10.1007/978-1-4020-9500-9_17
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