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An Overview on Tobacco Doubled Haploids

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Book cover Advances in Haploid Production in Higher Plants

Androgenesis is a process of redirection of normal pollen development towards the formation of haploid embryos and ultimately doubled-haploid plants. This review gives a historical overview of different procedures used to induce androgenesis in tobacco. The various factors that influence this developmental switch including growth of donor plants, pre-treatments, culture media, as well as cytological aspects of cell and tissue changes that occur during the induction and development of the microspore-derived embryos are described. Finally, this review will cover recent molecular data on mechanisms of embryogenic induction in cultured tobacco microspores

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References

  • Aionesei T, Touraev A, Heberle-Bors E (2005) Pathways to microspore embryogenesis. In: Palmer CE, Keller WA, Kasha KJ (eds) Haploids in Crop Improvement II. Springer, Berlin/Heidelberg/ New York, pp 11–34

    Chapter  Google Scholar 

  • Anagnostakis SL (1974) Haploid plants from tobacco—Enhancement with charcoal. Planta 115: 281–283

    Article  Google Scholar 

  • Benito-Moreno RM, Macke F, Hauser M-T, Alwen A, Heberle-Bors E (1988) Sporophytes and male gametophytes from in vitro cultured immature tobacco pollen. In: Cresti M, Jori P, Pacini E (eds) Sexual Reproduction in Higher Plants. Springer, Berlin/Heidelberg/New York, pp 137–142

    Google Scholar 

  • Binarova P, Straatman K, Hause B, Hause G, Van Lammeren AAM (1993) Nuclear DNA synthesis during the induction of embryogenesis in cultured microspores and pollen of Brassica napus L. Theor Appl Genet 87: 9–16

    Article  CAS  Google Scholar 

  • Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploids à partir détamines cultivées in vitro. Ann Physiol 9: 377–382

    Google Scholar 

  • Boutilier K, Fiers M, Liu C-M, Geest AHM (2005) Biochemical and molecular aspects of haploid embryogenesis, In: Palmer D, Keller W, Kasha K (eds) Haploids in Crop Improvement II. Springer, Heidelberg, pp 73–96

    Chapter  Google Scholar 

  • Clausen RE, Mann MC (1924) Inheritance in Nicotiana tabacum V. The occurrence of haploid plants in interspecific progenies. Proc Natl Acad Sci USA 10: 121–124

    Article  PubMed  CAS  Google Scholar 

  • Duncan EJ, Heberle E (1976) Effect of temperature shock on nuclear phenomena in microspores of Nicotiana tabacum and consequently on plantlet production. Protoplasma 90: 173–177

    Article  Google Scholar 

  • Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16: 109–118

    Article  Google Scholar 

  • Dunwell JM (1981) Stimulation of pollen embryo induction in tobacco by pretreatment of excised anthers in a water-saturated atmosphere. Plant Sci Lett 21: 9–13

    Article  Google Scholar 

  • Dunwell JM, Sunderland N (1974a) Pollen ultrastructure in anther cultures of Nicotiana tabacum I. Early stages of culture. J Exp Bot 25: 352–361

    Article  Google Scholar 

  • Dunwell JM, Sunderland N (1974b) Pollen ultrastructure in anther cultures of Nicotiana tabacum II. Changes associated with embryogenesis. J Exp Bot 25: 363–373

    Article  Google Scholar 

  • Dunwell JM, Sunderland N (1975) Pollen ultrastructure in anther cultures of Nicotiana tabacum III. The first sporophytic division. J Exp Bot 26: 240–252

    Article  Google Scholar 

  • Dunwell JM, Sunderland N (1976) Pollen ultrastructure in anther cultures of Datura innoxia. J Cell Sci 22: 469–501

    PubMed  CAS  Google Scholar 

  • Garrido D, Charvat B, Benito-Moreno RM, Alwen A, Vicente O, Heberle-Bors E (1991) Pollen culture for haploid plant production in tobacco. In: Negrutiu I, Gharti-Chhetri G (eds) A Laboratory Guide for Cellular Molecular Plant Biology. Birkhäuser, Basel, pp 59–69

    Google Scholar 

  • Garrido D, Eller N, Heberle-Bors E, Vicente O (1993) De novo transcription of specific messenger RNAs during the induction of tobacco pollen embryogenesis. Sex Plant Reprod 6: 40–45

    Article  Google Scholar 

  • Garrido D, Vicente O, Heberle-Bors E, Rodriguez-García MI (1995) Cellular changes during the acquisition of embryogenic potential in isolated pollen grains of Nicotiana tabacum. Protoplasma 186: 220–230

    Article  Google Scholar 

  • Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204: 497

    Article  Google Scholar 

  • Guha S, Maheshwari SC (1966) Cell division differentiation of embryos in the pollen grains of Datura in vitro. Nature 212: 97–98

    Article  Google Scholar 

  • Harada H, Kyo M, Imamura J (1988) The induction of embryogenesis in Nicotiana immature pollen in culture. In: Applications of plant cell tissue culture. Ciba Foundation Symposium, Wiley, Chichester, pp 59–74

    Chapter  Google Scholar 

  • Heberle-Bors E (1982) On the time of embryogenic pollen grain induction during sexual development of Nicotiana tabacum L plants. Planta 156: 402–406

    Article  Google Scholar 

  • Heberle-Bors E (1983) Induction of embryogenic pollen grains and sebsequent embryogenesis in Nicotiana tabacum L by treatments of the pollen donor plants with feminizing agents Physiol Plant 59: 67–72

    Article  Google Scholar 

  • Heberle-Bors E, Reinert J (1979) Androgenesis in isolated pollen cultures of Nicotiana tabacum: dependence upon pollen development. Protoplasma 99: 339–347

    Article  Google Scholar 

  • Heberle-Bors E, Reinert J (1980) Isolated pollen cultures and pollen dimorphism. Naturwiss 67: 311

    Article  Google Scholar 

  • Heberle-Bors E, Reinert J (1981) Environmental control and evidence for predetermination of pollen embryogenesis in Nicotiana tabacum pollen. Protoplasma 109: 249–255

    Article  Google Scholar 

  • Horner M, Street E (1978) Pollen dimorphism—origin and significance in pollen plant formation by anther culture. Ann Bot 42: 763–777

    Google Scholar 

  • Horner M, McComb JA, McComb AJ, Street HE (1977) Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J Exp Bot 28: 1365–1372

    Article  CAS  Google Scholar 

  • Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A (2007a) Transcriptional and metabolic profiles of stress- induced, embryogenic tobacco microspores. Plant Mol Biol 63: 137–149

    Article  CAS  Google Scholar 

  • Hosp J, Maraschin SF, Touraev A, Boutilier K (2007b) Functional genomics of microspore embryogenesis. Euphytica 158: 275–285

    Article  Google Scholar 

  • Imamura J, Harada H (1981) Stimulation of tobacco pollen embryogenesis by anaerobic treatments. Z Planzenphysiol 103: 259–263

    Google Scholar 

  • Imamura J, Okabe E, Kyo M, Harada H (1982) Embryogenesis and plantlet formation through direct culture of isolated pollen of Nicotiana tabacum cv Samsum and Nicotiana rustica cv Rustica. Plant Cell Physiol 23: 713–716

    Google Scholar 

  • Kohlenbach HW, Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Planzenphysiol 86: 463–472

    CAS  Google Scholar 

  • Konar RN (1963) A haploid tissue from the pollen of Ephedra foliate. Boiss Phytomorphology 13: 170–174

    Google Scholar 

  • Kyo M, Harada H (1985) Studies on conditions for cell division and embryogenesis in isolated pollen culture of Nicotiana rustica. Plant Physiol 79: 90–94

    Article  PubMed  CAS  Google Scholar 

  • Kyo M, Harada H (1986) Control of the developmental pathway of tobacco pollen in vitro. Planta 168: 427–432

    Article  CAS  Google Scholar 

  • Kyo M, Yamaji N, Yuasa Y, Maeda T, Fukui H (2002) Isolation of cDNA coding for NtEPb1-b3, marker proteins for pollen dedifferentiation in a tobacco pollen culture system. Plant Sci 163: 1055–1062

    Article  CAS  Google Scholar 

  • Kyo M, Hattori S, Yamaji N, Pechan P, Fukui H (2003) Cloning and characterization of cDNAs associated with the embryogenic dedifferentiation of tobacco immature pollen grains. Plant Sci 163: 1055–1062

    Article  Google Scholar 

  • Maraschin SF Priester W de Spaink HP Wang M (2005) Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective J Exp Bot 56: 1711–1726

    Article  PubMed  CAS  Google Scholar 

  • Milla SR, Levin JS, Lewis RS, Rufty RC (2005) RAPD and SCAR markers linked to an intro- gressed Gene Conditioning Resistance to Peronospora tabacina D.B Adam in tobacco. Crop Sci 45: 2346–2354

    Article  CAS  Google Scholar 

  • Murad L, Yoong Lim K, Christopodulou V, Matyasek R, Lichtenstein CP, Kovarik A, Leitch AR (2002) The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae). Am J Bot 89: 921–928

    Article  CAS  Google Scholar 

  • Nakata K, Tanaka M (1968) Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jpn J Genet 43: 65–71

    Article  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19: 389–404

    Google Scholar 

  • Nitsch JP (1972) Haploid plants from pollen. Z Pflanzenzüchtg 67: 3–18

    Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87

    Article  PubMed  Google Scholar 

  • Nitsch C, Norreel B (1973) Effet dun choc thermique sur le pouvoir embryogene du pollen de Datura innoxia cultive dans lanthere ou isole de lanthere CR Acad Sci Ser D 276: 303–306

    Google Scholar 

  • Pechan PM, Keller WA (1988) Identification of potentially embryogenic microspores in Brassica napus. Physiol Plant 74: 377–384

    Article  Google Scholar 

  • Raina SK, Irfan ST (1998) High frequency embryogenesis and plantlet regeneration from isolated microspores of indica rice. Plant Cell Rep 17: 957–962

    Article  CAS  Google Scholar 

  • Rashid A, Siddiqui AW, Reinert J (1981) Ultrastructure of embryogenic pollen of Nicotiana taba-cum var Badischer Burley. Protoplasma 107: 375–385

    Article  Google Scholar 

  • Ribarits A, Mamun AN, Li S, Resch T, Fiers M, Heberle-Bors E, Liu CM, Touraev A (2007) Combination of reversible male sterility and doubled haploid production by targeted inactivation of cytoplasmic glutamine synthetase in developing anthers and pollen. Plant Biotechnol J 5: 483–494

    Article  PubMed  CAS  Google Scholar 

  • Sunderland N (1971) Anther culture: a progress report. Sci Prog Oxf 59: 527–549

    Google Scholar 

  • Sunderland N (1978) Strategies on the improvement of yields in anther culture. In: Proceedings of Symposium on Plant Tissue Culture. Science Press, Peking, pp 65–86

    Google Scholar 

  • Sunderland N, Dunwell JM (1977) Anther and pollen culture. In: Street HE (ed) Plant Tissue and Cell Culture. Oxford, Blackwell, pp 223–265

    Google Scholar 

  • Sunderland N, Wicks FM (1969) Cultivation of haploid plants from tobacco pollen. Nature 224: 1227

    Article  Google Scholar 

  • Sunderland N, Wicks FM (1971) Embryoid formation in pollen grains of Nicotiana tabacum. J Exp Bot 22: 213–226

    Article  Google Scholar 

  • Tai GCC (2005) Haploids in the improvement of Solanaceous species. In: Palmer CE, Keller WA, Kasha KJ (eds) Haploids in Crop Improvement II. Springer, Berlin/Heidelberg, pp 173–190

    Chapter  Google Scholar 

  • Touraev A, Heberle-Bors E (2003) Anther and microspore culture in tobacco. In: Maluszynski M et al. (eds) Doubled Haploid Production in Crop Plants. Dordrecht, Netherlands: Kluwer Academic Publishers pp 223–228

    Google Scholar 

  • Touraev A, Ilham A, Vicente O, Heberle-Bors E (1996a) Stress-induced microspore embryogen-esis in tobacco: an optimized system for molecular studies. Plant Cell Rep 15: 561–565

    Article  CAS  Google Scholar 

  • Touraev A, Pfosser M, Vicente O, Heberle-Bors E (1996b) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of micro-spore/pollen embryogenesis. Planta 200: 144–152

    Article  CAS  Google Scholar 

  • Touraev A, Vicente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2: 285–303

    Article  Google Scholar 

  • Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35: 53–109

    Article  Google Scholar 

  • Tulecke WR (1953) A tissue derived from the pollen of Ginkgo biloba. Science 117: 599–600

    Article  PubMed  CAS  Google Scholar 

  • Tulecke WR (1959) The pollen cultures of C.D. LaRue: a tissue from the pollen of Taxus. Bull Torrey Bot Club 86: 283–289

    Article  Google Scholar 

  • Tulecke WR, Sehgal N (1963) Cell proliferation from the pollen of Torreya nucifera. Contrib Boyce Thompson Inst 22: 153–163

    Google Scholar 

  • Wenzel G, Thomas E (1974) Observations on the growth in culture of anthers of Secale cereale. Z Pflanzenzüchtg 72: 89–94

    Google Scholar 

  • Wernsman EA (1993) Varied roles for the haploid sporophyte in plant improvement. In: Stalker HT, Murphy JP (eds) Plant Breeding in the 1990s. CAB International, Wallingford, pp 461–484

    Google Scholar 

  • Zaki MAM, Dickinson HG (1990) Structural changes during the first divisions of embryos resulting from anther and microspore culture in Brassica napus. Protoplasma 156: 149–162

    Article  Google Scholar 

  • Zarsky V, Garrido D, Rihova L, Tupy J, Vicente O, Heberle-Bors E (1992) Derepression of the cell cycle by starvation is involved in the induction of tobacco pollen embryogenesis. Sex Plant Reprod 5: 189–194

    Article  Google Scholar 

  • Zarsky V, Garrido D, Eller N, Tupy J, Vicente O, Schöffl F, Heberle-Bors E (1995) The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation. Plant Cell Environ 18: 139–147

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Max F Perutz Laboratories, Dr. Bohrgasse 9/4, Vienna, 1030, Austria

    K. Belogradova, I. Lewicka, E. Heberle-Bors & A. Touraev

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  1. K. Belogradova
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  2. I. Lewicka
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  3. E. Heberle-Bors
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  4. A. Touraev
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Correspondence to A. Touraev .

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Editors and Affiliations

  1. Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9/4, Vienna, 1030, Austria

    Alisher Touraev

  2. BioHybrids International Ltd. Earley, Earley, Reading, RG6 5FY, United Kingdom

    Brain P. Forster

  3. Department of Applied BiologyDepartment of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, FI-00014, Finland

    S. Mohan Jain

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Belogradova, K., Lewicka, I., Heberle-Bors, E., Touraev, A. (2009). An Overview on Tobacco Doubled Haploids. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_5

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