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Doubled Haploids via Gynogenesis

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Advances in Haploid Production in Higher Plants

Gynogenic haploid regeneration is an alternative procedure for haploid induction used in several agronomically important species such as sugar-beet and onion. At inoculation, developmental stage of ovules is frequently immature, but in contrast to androgenesis, in vitro maturation of embryo sac occurs. The egg cells were found to be the predominant source of haploid embryos while antipodal or synergid origin has been also proposed for some species. For majority of species triggering factors promoting haploid embryogenesis are not apparent, but media constituents such as phytohormons and carbohydrates evidently have some role in reprogramming. The majority of regenerants obtained via gynogenesis are haploid, for optimization of treatments large experimental units are proposed and alternative to the use of antimitotic agents spontaneous duplication via adventitious regeneration is proposed. Major characteristics of successful protocols are listed

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References

  • Alan AR, Mutschler MA, Brants A, Cobb E, Earle ED (2003) Production of gynogenic plants from hybrids of Allium cepa L. and A. roylei Stearn. Plant Sci 165: 1201–1211

    Article  CAS  Google Scholar 

  • Alan AR, Brants A, Cobb E, Goldschmied PA, Mutschler MA, Earle ED (2004) Fecund gyno-genic lines from onion (Allium cepa L.) breeding materials. Plant Sci 167: 1055–1066

    Article  CAS  Google Scholar 

  • Alan AR, Lim W, Mutschler MA, Earle ED (2007) Complementary strategies for ploidy manipulations in gynogenic onion (Allium cepa L.) Plant Sci 173: 25–31

    Article  CAS  Google Scholar 

  • Bara ski R (1996) In vitro gynogenesis in red beet (Beta vulgaris L): Effects of ovule culture conditions. Acta Soc Bot Pol 65: 57–60

    Google Scholar 

  • Bhojwani SS, Chaturvedi R, Raina A (2003) Tissue culture of woody species. In: Bhojwani SS, Soh WY (eds) Agrobiotechnology and plant tissue culture, Science Publishers, Enfield, Plymouth, pp 57–80

    Google Scholar 

  • Blakeslee AF, Belling J, Farnham ME.; Bergner AD (1922) A haploid mutant in the Jimson weed, ‘Datura stramonium’. Science 55: 646–647

    Article  PubMed  Google Scholar 

  • Bohanec B, JakÅ¡e M (1999) Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Plant Cell Rep 18: 737–742

    Article  CAS  Google Scholar 

  • Bohanec B., JakÅ¡e M, Ihan A, Javornik B (1995) Studies of gynogenesis in onion (Allium cepa L.) — induction procedures and genetic analysis of regenerants. Plant Sci 104: 215–224

    Article  CAS  Google Scholar 

  • Bohanec B, JakÅ¡e M, Havey MJ (2003) Genetic analyses of gynogenetic haploid production in onion. J Am Soc Hortic Sci 128: 571–574

    Google Scholar 

  • Campion B, Azzimonti MT, Vicini E, Schiavi M, Falavigna A (1992) Advances in haploid plant induction (Allium cepa L.) through in vitro gynogenesis. Plant Sci 86: 97–104

    Article  Google Scholar 

  • Castillo AM, Cistué L (1993) Production of gynogenic haploids of Hordeum vulgare L. Plant Cell Rep 12: 139–143

    CAS  Google Scholar 

  • Chand S, Basu P (1998) Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L. Plant Cell Rep 17: 302–305

    Article  CAS  Google Scholar 

  • Cohat J (1994) Production of gynogenetic plants by in vitro culture of flower buds in shallot (Allium cepa L. var aggregatum). Agronomie 14: 299–304

    Article  Google Scholar 

  • Doctrinal M, Sangwan RS, Sangwan-Norreel BS (1989) In vitro gynogenesis in Beta vulgaris L. — effects of plant growth regulators, temperature, genotypes and season. Plant Cell Tiss Org Cult 17: 1–12

    Google Scholar 

  • Eeckhaut T, Werbrouck S, Dendauw J, Van Bockstaele E, Debergh P (2001) Induction of homozygous Spathiphyllum wallisii genotypes through gynogenesis. Plant Cell Tiss Org Cult 67: 181–189

    Article  CAS  Google Scholar 

  • Ferrant V, Bouharmont J (1994): Origin of gynogenetic embryos of Beta vulgaris L. Sex Plant Reprod 7: 12–16

    Google Scholar 

  • Gelebart P, San LH (1987) Production of haploid plants of sunflower (Helianthus annuus L) by in vitro culture of non-fertilized ovaries and ovules. Agronomie 7: 81–86

    Article  Google Scholar 

  • Gemes-Juhasz A, Balogh P, Ferenczy A, Kristof Z (2002) Effect of optimal stage of female game-tophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Rep 21: 105–111

    Article  Google Scholar 

  • Germana M (2006) Doubled haploid production in fruit crops. Plant Cell Tiss Org Cult 86: 131–146 Geoffriau E, Kahane R, Martin-Tanguy J (2006) Polyamines are involved in the gynogenesis process in onion. Physiol Plant 127: 119–129

    Article  Google Scholar 

  • Geoffriau E, Kahane R, Martin-Tanguy J (2006) Polyamines are involved in the gynogenesis process in onion. Physiol Plant 127: 119–129

    Article  CAS  Google Scholar 

  • Geoffriau E, Kahane R, Rancillac M (1997) Variation of gynogenesis ability in onion (Allium cepa L). Euphytica 94: 37–44

    Article  Google Scholar 

  • Gugsa, Likyelesh; Sarial, Ashok; Lörz, Horst; Kumlehn, Jochen (2006) Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter. Plant Cell Rep 25: 1287–1293

    Article  PubMed  CAS  Google Scholar 

  • Guo F, Jia Xj, Chen LX (1982) Induction of plantlets from isolated ovules of Hevea brasiliensis. Hereditas 4: 27–28

    Google Scholar 

  • Gurel S, Gurel E, Kaya Z (2000) Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.) Plant Cell Rep 19: 1155–1159

    Article  CAS  Google Scholar 

  • Huang QT, Yang H Y, Zhou C (1982) Embryological observations on ovary culture of unpollinated young flowers in Hordeum vulgare L. Acta Bot Sin 24: 295–300

    Google Scholar 

  • Jähne A, Lazzeri PA, Jagergussen M, Lörz H (1991) Plant-regeneration from embryogenic cell-suspensions derived from anther cultures of barley (Hordeum vulgare L.) Theor Appl Genet 82: 74–80

    Article  Google Scholar 

  • JakÅ¡e M, Havey MJ, Bohanec B (2003) Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos. Plant Cell Rep 21: 905–910

    PubMed  Google Scholar 

  • Javornik B, Bohanec B, Campion B (1998) Second cycle gynogenesis in onion, Allium cepa L, and genetic analysis of the plants. Plant Breed 117: 275–278

    Article  Google Scholar 

  • Kasha KJ (2005) Chromosome doubling and recovery of doubled haloid plants. In: Palmer CE, Keller WA, Kasha KJ (eds) Biotechnology in agriculture and forestry, Vol. 56. Haploids in crop improvement, Springer, Berlin/Heidelberg, pp 123–152

    Google Scholar 

  • Kashin AS, Blyudneva EA, Silkin MA (2000) Megagamete activation and regulation of in vitro embryogenesis in unpollinated millet ovaries. Russ J Plant Physiol 47: 260–269

    CAS  Google Scholar 

  • Kim S, Binzel ML, Yoo KS, Park S, Pike LM (2004) Pink (P), a new locus responsible for a pink trait in onions (Allium cepa) resulting from natural mutations of anthocyanidin synthase. Mol Genet Genomics 272: 18–27

    PubMed  CAS  Google Scholar 

  • Laksmi-Sita G (1997) Gynogenic haloids in vitro. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Vol. 5. Kluwer, Dordrecht, pp 175–193

    Google Scholar 

  • Lux H, Herrmann L, Wetzel C (1990) Production of haploid sugar-beet (Beta vulgaris L) by cul-turing unpollinated ovules. Plant Breed 104: 177–183

    Article  Google Scholar 

  • Martinez LE, Aguero CB, Lopez ME, Galmarini CR (2000) Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Plant Sci 156: 221–226

    Article  PubMed  CAS  Google Scholar 

  • Matzk F, Prodanovic S, Baumlein H, Schubert I (2005) The inheritance of apomixis in Poa prat-ensis confirms a five locus model with differences in gene expressivity and penetrance. Plant Cell 17: 13–24

    Article  PubMed  CAS  Google Scholar 

  • Mdarhri-Alaoui M, Saidi N, Chlyah A, Chlyah H (1998) Green haploid plant formation in durum wheat through in vitro gynogenesis. CR Acad Sci Serie III — Life 32: 25–30

    Google Scholar 

  • Metwally EI, Moustafa SA, El-Sawy BI, Haroun SA, Shalaby TA (1998) Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo. Plant Cell Tiss Org Cult 52: 117–121

    Article  CAS  Google Scholar 

  • Meynet J, Sibi M (1984) Haploid plants from in vitro culture of unfertilized ovules in Gerbera jamesonii. Z Pflanzenzucht 93: 78–85

    Google Scholar 

  • Michalik B, Adamus A, Nowak E (2000) Gynogenesis in Polish onion cultivars. J Plant Physiol 156: 211–216

    CAS  Google Scholar 

  • Miyoshi K, Asakura N (1996) Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Rep 16: 1–5

    Article  CAS  Google Scholar 

  • Mol R (1992) In vitro gynogenesis in Melandrium album — from partenogenetic embryos to mix-oploid plants. Plant Sci 8: 261–269

    Article  Google Scholar 

  • Mukhambetzhanov SK (1997) Culture of nonfertilized female gametophytes in vitro. Plant Cell Tiss Org Cult 48: 111–119

    Article  Google Scholar 

  • Muren RC (1989) Haploid plant production from unpollinated ovaries in onion. Hortscience 24 (5): 833–834

    Google Scholar 

  • Musial K, Bohanec B, Przywara L (2001) Embryological study on gynogenesis in onion (Allium cepa L.). Sex Plant Reprod 13: 335–341

    Article  Google Scholar 

  • Musial K, Bohanec B, Jakse M, Przywara L (2005) The development of onion (Allium cepa L.) embryo sacs in vitro and gynogenesis induction in relation to flower size. In vitro Cell Dev Biol — Plant 41: 446–452

    Article  Google Scholar 

  • Nitch JP, Nitch C (1969) Haploid plants from pollen grains. Science 163: 85–87

    Article  Google Scholar 

  • Palmer CE, Keller WA (2005) Overview of haploidy. In: Nagata T, Lorz H, Widholm JM (eds) Biotechnology in agriculture and forestry — Haploids in crop improvement II. Springer, Berlin/ Heidelberg, pp 3–10

    Chapter  Google Scholar 

  • Pedersen HC, Keimer B (1996) Haploidy in sugar beet (Beta vulgaris L.). Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Vol. 3. Kluwer, Dordrecht, pp 17–36

    Google Scholar 

  • Ponce M, Martinez L, Galmarini C (2006) Influence of CCC, putrescine and gellam gum concentration on gynogenic embryo induction in Allium cepa. Biol Plant 50: 425–428

    Article  CAS  Google Scholar 

  • Puddephat IJ, Robinson HT, Smith BM, Lynn J (1999) Influence of stock plant pretreatment on gynogenic embryo induction from flower buds of onion. Plant Cell Tiss Org Cult 57: 145–148

    Article  Google Scholar 

  • Ramsay JL, Galitz DS, Lee CW (2003) Basal medium and sucrose concentration influence regeneration of easter lily in ovary culture. Hortscience 38: 404–406

    Google Scholar 

  • Rongbai Li, Pandey MP, Garg GK, Pandey SK, Dwivedi DK, Ashima (1998) Development of a technique for in vitro unpollinated ovary culture in rice, Oryza sativa L. Euphytica 104: 159–166

    Article  Google Scholar 

  • Rongbai Li, Pandey MP, Pandey SK, Dwivedi, DK (1999) Agro-morphological characterization of ovary culture-derived plants of rice (Oryza sativa L.). Euphytica 106: 197–203

    Article  Google Scholar 

  • San Noeum LH (1976) Haploides d'hordeum vulgare L. par culture in vitro non fecondes. Ann Amelior Plant 26: 751–754

    Google Scholar 

  • San Noeum LH (1979) In vitro induction of gynogenesis in higher plants. Broadening genetic base of crops. Proceeding of the conference, Wageningen, The Netherlands, pp 327–329

    Google Scholar 

  • Sibi ML, Kobaissi A, Shekafandeh A (2001) Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica 122: 351–359

    Article  Google Scholar 

  • Sitbon M (1981) Production of haploid Gerbera jamesonii plants by in vitro culture of unfertilized ovules. Agronomie 1: 807–812

    Article  Google Scholar 

  • Sulistyaningsih E, Yamashita K, Tashiro Y (2002) Haploid induction from F-1 hybrids between CMS shallot with Allium galanthum cytoplasm and common onion by unpollinated flower culture. Euphytica 125: 139–144

    Article  CAS  Google Scholar 

  • Å kof, Bohanec, Kastelec, Luthar Z (2007) Spontaneous induction of tetraploidy in hop using adventitious shoot regeneration method. Plant Breed 126: 416–421

    Article  Google Scholar 

  • Thomas TD, Bhatnagar AK, Razdan MK, Bhojwani SS (1999) A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L. Euphytica 110: 169–173

    Article  Google Scholar 

  • Tosca A, Arcara L, Frangi P (1999) Effect of genotype and season on gynogenesis efficiency in Gerbera. Plant Cell Tiss Org Cult 59: 77–80

    Article  Google Scholar 

  • Truong-Andre I, Demarly Y (1984) Obtaining plants by in vitro culture of unfertilized maize ovaries (Zea mays L.) and preliminary studies on the progeny of a gynogenetic plant. Z Pflanzenzucht 92: 309–320

    Google Scholar 

  • VanGeyt J, Speckmann GJ, Halluin KD, Jacobs M (1987) In vitro induction of haploid plants from unpollinated ovules and ovaries of the sugar beet (Beta vulgaris L.). Theor Appl Genet 73: 920–925

    Article  CAS  Google Scholar 

  • Wu BJ, Chen KC (1982) Cytological and embryological studies on haploid plant production from cultured unpollinated ovaries of Nicotiana tabacum L. Acta Bot Sin 24: 125–129

    Google Scholar 

  • Yadegari R, Drews GN (2004) Female gametophyte development. Plant Cell 16: S133–S141

    Article  PubMed  CAS  Google Scholar 

  • Yang H Y, Zhou C (1982) In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor Appl Genet 63: 97–104

    Article  Google Scholar 

  • Yang H, Zhou C, Cai D, Yan H, Wu Y, Chen X (1986) In vitro culture of unfertilized ovules in Helianthus annuus L. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. Springer, Berlin, pp 182–191

    Google Scholar 

  • Zhou C, Yang H, Tian H, Liu Z, Yan H (1986) In vitro culture of unpollinated ovaries in Oryza sativa L. In: Hu H, Yang H (eds) Haploids of higher plants in vitro. Springer, Berlin, pp 167–181

    Google Scholar 

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Authors and Affiliations

  1. University of Ljubljana Biotechnical Faculty, Jamnikarjeva 101, Ljubljana, 1000, Slovenia

    B. Bohanec

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  1. B. Bohanec
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Correspondence to B. Bohanec .

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Editors and Affiliations

  1. Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9/4, Vienna, 1030, Austria

    Alisher Touraev

  2. BioHybrids International Ltd. Earley, Earley, Reading, RG6 5FY, United Kingdom

    Brain P. Forster

  3. Department of Applied BiologyDepartment of Applied Biology, University of Helsinki, Latokartanonkaari 5, Helsinki, FI-00014, Finland

    S. Mohan Jain

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Bohanec, B. (2009). Doubled Haploids via Gynogenesis. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_2

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