Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abbot, A. (2003) Biology's new dimension. Nature, 424: 870–872.
Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thomson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol., 227: 271–278.
Amit, M., Shariki, C., Margulets, V., and Itskovitz-Eldor, J. (2004) Feeder layer- and serum-free culture of human embryonic stem cells. Biol. Reprod., 70: 837–845.
Anderson, D.G., Burdick, J.A., and Langer, R. (2004) Materials science. Smart biomaterials. Science, 305: 1923–1924.
Brimble, S.N., Zeng, X., Weiler, D.A., Luo, Y., Liu, Y., Lyons, I.G., Freed, W.J., Robins, A.J., Rao, M.S., and Schulz, T.C. (2004) Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001. Stem Cells Dev., 13: 585–597.
Burdick, J.A., Chung, C., Jia, X., Randolph, M.A., and Langer, R. (2005) Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks. Biomacromolecules, 6: 386–391.
Burdick, J.A., Khademhosseini, A., and Langer, R. (2004) Fabrication of gradient hydrogels using a microfluidics/photopolymerization process. Langmuir, 20: 5153–5156.
Buzzard, J.J., Gough, N.M., Crook, J.M., and Coleman, A. (2004) Karyotype of human ES cells during extended culture. Nat. Biotechnol., 22: 381–382.
Carpenter, M.K., Chunhui, Xu., Daight, C.A., Antosiewiczm J.E., and Thomsonm J.A. (2003) Protocols for the maintenance of human embryonic stem cells. In: Human Embryonic Stem Cells, Chiu, A. and Rao, M.S. (eds.), Humana Press Totowa, NJ.
Dar, A., Shachar, M., Leor, J., and Cohen, S. (2002) Optimization of cardiac cell seeding and distribution in 3D porous alginate scaffolds. Biotechnol. Bioeng., 80: 305–312.
Draper, J.S., Smith, K., Gokhale, P., Moore, H.D., Maltby, E., Johnson, J., Meisner, L., Zwaka, T.P., Thomson, J.A., and Andrews, P.W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol., 22: 53–54.
Evanko, S.P. and Wight, T.N. (1999) Intracellular localization of hyaluronan in proliferating cells. J. Histochem. Cytochem., 47: 1331–1342.
Ferreira, L., Gil, M.H., and Dordick, J.S. (2002) Enzymatic synthesis of dextran-containing hydrogels. Biomaterials, 23: 3957–3967.
Gerecht-Nir, S., Cohen, S., and Itskovitz-Eldor, J. (2004a) Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells. Biotechnol. Bioeng., 86: 493–502.
Gerecht-Nir, S., Cohen, S., Ziskind, A., and Itskovitz-Eldor, J. (2004b) Bioreactor cultivation enhances the efficiency of human embryoid body (hEB) formation and differentiation. Biotechnol. Bioeng., 88: 313–320.
Gerecht-Nir, S., Radisic, M., Park, H., Boublik, J., Cannizzaro, C., Langer, R., and Vunjak-Novakovic, G. (2006) Biophysical regulation of cardiogenesis. Int. J. Dev. Biol., 50: 233–243.
Gerecht-Nir, S., Burdick, J.A., Ferreira, L.S., Townsend, S.A., Langer, R., and Vunjak-Novakovic, G. Propagation of undifferentiated human embryonic stem cells in hyaluronic acid hydrogels. PNAS (in review).
Glicklis, R., Shapiro, L., Aqbaria, R., Merchuk, J.C., and Cohen, S. (2000) Hepatocyte behavior within three-dimensional porous alginate scaffolds. Biotechnol. Bioeng., 67: 344–353.
Hoffman, L.M. and Carpenter, M.K. (2005) Characterization and culture of human embryonic stem cells. Nat. Biotechnol., 23: 699–708.
Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., Soreq, H., and Benvenisty, N. (2000) Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med., 6: 88–95.
Jacks, T. and Weinberg, R.A. (2002) Taking the study of cancer cell survival to a new dimension. Cell, 111: 923–925.
Khademhosseini, A., Eng, G., Yeh, J., Fukuda, J., Blumling, J. 3rd., Langer, R., and Burdick, J.A. (2006) Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapment. Biomed. Mater. Res. A., Jun 20; [Epub ahead of print].
Leor, J., Aboulafia-Etzion, S., Dar, A., Shapiro, L., Barbash, I., Battler, A., Granot, Y., and Cohen, S. (2000) Bioengineered cardiac grafts: a new approach to repair the infarcted myocardium. Circulation, 102: 56–61.
Levenberg, S., Golub, J.S., Amit, M., Itskovitz-Eldor, J., and Langer, R. (2002) Endothelial cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A, 99: 4391–4396.
Levenberg, S., Huang, N.F., Lavik, E., Rogers, A.B., Itskovitz-Eldor, J., and Langer, R. (2003) Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc. Natl. Acad. Sci. U.S.A, 100: 12741–12746.
Levenberg, S., Burdick, J.A., Kraehenbuehl T., and Langer R. (2005) Neurotrophin-induced differentiation of human embryonic stem cells on three-dimensional polymeric scaffolds. Tissue Eng., 11: 506–512.
Lutolf, M.P. and Hubbell, J.A. (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat. Biotechnol., 23: 47–55.
Mitalipova, M.M., Rao, R.R., Hoyer, D.M., Johnson, J.A., Meisner, L.F., Jones, K.L., Dalton, S., and Stice, S.L. (2005) Preserving the genetic integrity of human embryonic stem cells. Nat. Biotechnol., 23: 9–20.
Postovit, L.M., Seftor, E.A., Seftor, R.E., and Hendrix, M.J. (2006) A three-dimensional model to study the epigenetic effects induced by the microenvironment of human embryonic stem cells. Stem Cells 24: 501–505.
Proetzel, G. and Wiles, M.V. (2002) The use of a chemically defined media for the analyses of early development in ES cells and mouse embryos. Methods Mol. Biol., 185: 17–26.
Radisic, M., Malda, J., Epping, E., Geng, W., Langer, R., and Vunjak-Novakovic, G. (2006) Oxygen gradients correlate with cell density and cell viability in engineered cardiac tissue. Biotechnol. Bioeng., 93: 332–343.
Radisic, M., Park, H., Gerecht-Nir, S., Cannizzaro, C., Langer, R., and Vunjak-Novakovic, G. Biomimetic approach to cardiac tissue engineering. Philosophical Transactions of the Royal Society of London (in press).
Richards, M., Fong, C.Y., Chan, W.K., Wong, P.C., and Bongso, A. (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat. Biotechnol., 20: 933–936.
Rosler, E.S., Fisk, G.J., Ares, X., Irving, J., Miura, T., Rao, M.S., and Carpenter, M.K. (2004) Long-term culture of human embryonic stem cells in feeder-free conditions. Dev. Dyn., 229: 259–274.
Schatten, G., Smith, J., Navara, C., Park, J.H., and Pedersen, R. (2005) Culture of human embryonic stem cells. Nat. Methods, 2: 455–463.
Shapiro, L. and Cohen, S. (1997) Novel alginate sponges for cell culture and transplantation. Biomaterials, 18: 583–590.
Stojkovic, P., Lako, M., Przyborski, S., Stewart, R., Armstrong, L., Evans, J., Zhang, X., and Stojkovic M. (2005) Human-serum matrix supports undifferentiated growth of human embryonic stem cells. Stem Cells, 23: 895–902.
Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147.
Toole, B.P. (2001) Hyaluronan in morphogenesis. Semin. Cell Dev. Biol., 12: 79–87.
Wang, L., Li, L., Menendez, P., Cerdan, C., and Bhatia, M. (2005) Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development. Blood, 105: 4598–4603.
Xu, C., Inokuma, M.S., Denham, J., Golds, K., Kundu, P., Gold, J.D., and Carpenter, M.K. (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol., 19: 971–974.
Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O'Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells, 23: 315–323.
Xu, R.H., Peck, R.M., Li, D.S., Feng, X., Ludwig, T., and Thomson, J.A. (2005) Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat. Methods, 2: 185–190.
Zmora, S., Glicklis, R., and Cohen, S. (2002) Tailoring the pore architecture in 3-D alginate scaffolds by controlling the freezing regime during fabrication. Biomaterials, 23: 4087–4094.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this chapter
Cite this chapter
Gerecht, S., Burdick, J.A., Cannizzaro, C., Vunjak-Novakovic, G. (2007). Three-dimensional Culture of Human Embryonic Stem Cells. In: Masters, J.R., Palsson, B.O., Thomson, J.A. (eds) Human Cell Culture. Human Cell Culture, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5983-4_9
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5983-4_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5982-7
Online ISBN: 978-1-4020-5983-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)