Skip to main content

Characterization and Differentiation of Human Embryonic Stem Cells

  • Chapter
  • 1464 Accesses

Part of the book series: Human Cell Culture ((HUCC,volume 6))

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma, 3: 347–361.

    Article  PubMed  CAS  Google Scholar 

  • Cooper, S., Bennett, W., Andrade, J., Reubinoff, B.E., Thomson, J., and Pera, M.F. (2002) Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells.Anat., 200: 259–265.

    Article  CAS  Google Scholar 

  • Cowan, C.A., Klimanskaya, I., McMahon, J., Atienza, J., Witmyer, J., Zucker, J.P., Wang, S., Morton, C.C., McMahon, A.P., Powers, D., and Melton, D.A. (2004) Derivation of embryonic stem-cell lines from human blastocysts. N. Engl.J.;Med., 350: 1353–1356.

    Article  PubMed  CAS  Google Scholar 

  • D'Amour, K.A., Agulnick, A.D., Eliazer, S., Kelly, O.G., Kroon, E., and Baetge, E.E. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol., 23: 1534–1541.

    Article  PubMed  Google Scholar 

  • Draper, J.S., Smith, K., Gokhale, P., Moore, H.D., Maltby, E., Johnson, J., Meisner, L., Zwaka, T.P., Thomson, J.A., and Andrews, P.W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol., 22: 53–54.

    Article  PubMed  CAS  Google Scholar 

  • Enver, T., Soneji, S., Joshi, C., Brown, J., Iborra, F., Orntoft, T., Thykjaer, T., Maltby, E., Smith, K., Dawud, R.A.et-al. (2005) Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells. Hum. Mol. Genet., 14: 3129–3140.

    Article  PubMed  CAS  Google Scholar 

  • Herszfeld, D., Wolvetang, E., Langton-Bunker, E., Chung, T.L., Filipczyk, A.A., Houssami, S., Jamshidi, P., Koh, K., Laslett, A.L., Michalska, A.,et-al. (2006) CD30 is a survival factor an biomarker for transformed human pluripotent stem cells. Nat. Biotechnol., 24: 351–357.

    Article  PubMed  CAS  Google Scholar 

  • Hyslop, L., Stojkovic, M., Armstrong, L., Walter, T., Stojkovic, P., Przyborski, S., Herbert, M., Murdoch, A., Strachan, T., and Lako, M. (2005) Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Stem Cells, 23: 1035–1043.

    Article  PubMed  CAS  Google Scholar 

  • Johansson, B.M. and Wiles, M.V. (1995) Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development. Mol. Cell. Biol., 15: 141–151.

    PubMed  CAS  Google Scholar 

  • Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., and Solter, D. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J., 2: 2355–2361.

    PubMed  CAS  Google Scholar 

  • Kannagi, R., Levery, S.B., Ishigami, F., Hakomori, S., Shevinsky, L.H., Knowles, B.B., and Solter, D. (1983) New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3.J.;Biol. Chem., 258: 8934–8942.

    PubMed  CAS  Google Scholar 

  • Kerjaschki, D., Poczewski, H., Dekan, G., Horvat, R., Balzar, E., Kraft, N., and Atkins, R.C. (1986) Identification of a major sialoprotein in the glycocalyx of human visceral glomerular epithelial cells.J.;Clin. Invest., 78: 1142–1149.

    Article  PubMed  CAS  Google Scholar 

  • Laslett, A.L., Filipczyk, A.A., and Pera, M.F. (2003) Characterization and culture of human embryonic stem cells. Trends Cardiovasc. Med., 13: 295–301.

    Article  PubMed  CAS  Google Scholar 

  • Laslett, A.L., Grimmond, S., Gardiner, B., Stamp, L., Lin, A., Hawes, S.M., Wormald, S., Nikolic-Paterson, D., Haylock, D., and Pera, M.F. (2007) Transcriptional analysis of early lineage commitment in human embryonic stem cells. BMC Dev. Biol., 7: 12.

    Article  PubMed  Google Scholar 

  • Lower, R., Lower, J., and Kurth, R. (1996) The viruses in all of us: characteristics and biological significance of human endogenous retrovirus sequences. Proc. Natl. Acad. Sci. USA, 93: 5177–5184.

    Article  PubMed  CAS  Google Scholar 

  • Ng, E.S., Davis, R.P., Azzola, L., Stanley, E.G., and Elefanty, A.G. (2005) Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation. Blood, 106: 1601–1603.

    Article  PubMed  CAS  Google Scholar 

  • Oka, M., Tagoku, K., Russell, T.L., Nakano, Y., Hamazaki, T., Meyer, E.M., Yokota, T., and Terada, N. (2002) CD9 is associated with leukemia inhibitory factor-mediated maintenance of embryonic stem cells. Mol. Biol. Cell, 13: 1274–1281.

    Article  PubMed  CAS  Google Scholar 

  • Pera, M.F., Andrade, J., Houssami, S., Reubinoff, B., Trounson, A., Stanley, E.G., Ward-van Oostwaard, D., and Mummery, C. (2004) Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin.J.;Cell Sci., 117: 1269–1280.

    Article  PubMed  CAS  Google Scholar 

  • Pera, M.F., Blasco-Lafita, M.J., Cooper, S., Mason, M., Mills, J., and Monaghan, P. (1988) Analysis of cell-differentiation lineage in human teratomas using new monoclonal antibodies to cytostructural antigens of embryonal carcinoma cells. Differentiation, 39: 139–149.

    Article  PubMed  CAS  Google Scholar 

  • Pera, M.F., Cooper, S., Mills, J., and Parrington, J.M. (1989) Isolation and characterization multipotent clone of human embryonal carcinoma cells. Differentiation, 42: 10–23.

    Article  PubMed  CAS  Google Scholar 

  • Pera, M.F., Filipczyk, A.A., Hawes, S.M., and Laslett, A.L. (2003) Isolation, characterization, and differentiation of human embryonic stem cells. Meth. Enzymol., 365: 429–446.

    Article  PubMed  CAS  Google Scholar 

  • Pinkel, D. and Albertson, D.G. (2005) Comparative genomic hybridization. Annu. Rev. Genomics Hum. Genet., 6: 331–354.

    Article  PubMed  CAS  Google Scholar 

  • Reubinoff, B.E., Itsykson, P., Turetsky, T., Pera, M.F., Reinhartz, E., Itzik, A., and Ben-Hur, T. (2001) Neural progenitors from human embryonic stem cells. Nat. Biotechnol., 19: 1134–1140.

    Article  PubMed  CAS  Google Scholar 

  • Son, Y.S., Park, J.H., Kang, Y.K., Park, J.S., Choi, H.S., Lim, J.Y., Lee, J.E., Lee, J.B., Ko, M.S., Kim, Y.S.,et-al. (2005) Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation. Stem Cells, 23: 1502–1513.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this chapter

Cite this chapter

Laslett, A.L., Lin, A., Pera, M.F. (2007). Characterization and Differentiation of Human Embryonic Stem Cells. In: Masters, J.R., Palsson, B.O., Thomson, J.A. (eds) Human Cell Culture. Human Cell Culture, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5983-4_3

Download citation

Publish with us

Policies and ethics