Abstract
Cell cultures that can produce multiple products of commercial interest simultaneously have the potential to overcome economic barriers to commercialization. This paper reviews several strategies (elicitation, immobilization, medium optimization, differentiation, metabolic engineering, bioreactor considerations) for enhancing valuable product synthesis in plant cell cultures, with a focus on the production of Taxol from Taxus cell cultures (our laboratory research focus). While plants are capable of synthesizing valuable products (secondary metabolites), often undifferentiated tissue cultures cannot produce significant amounts of the target compound without the use of enhancement strategies. For example, cell immobilization cannot only protect cells from shear, but also promote secondary metabolism, in part due to increased cell interactions. The cell culture medium can be easily manipulated by changing sugar composition, type, and concentration of plant growth hormones, and the levels of phosphate and nitrogen. A two-stage process that promotes growth in the first stage and production in the second stage is often employed. Elicitation of enzyme systems can be used to achieve high levels of production. Methyl jasmonate is a signal transducer that has been shown to influence numerous plant species, including Taxus. Often, the synergistic application of these enhancement strategies is necessary to achieve acceptable product yields. There are also several issues to consider when designing a reactor for secondary metabolite production: shear stress, gas phase composition, and oxygen delivery. The generic strategies used to enhance secondary metabolite production in plant cell cultures can also be applied to increase hydrogen and valuable product yields in bacterial and algal systems.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bailey, J., 1991, Toward a science of metabolic engineering, Science, 252:1668–1675.
Bruce, N., French, C., Hailes, A., Long, M., and Rathbone, D., 1995, Engineering pathways for transformations of morphine alkaloids, Trends in Biotechnology, 13:200–205.
Constabel, F., Gaudet-LaPrairie, P., Kurz, W., and Kutney, J., 1982, Alkaloid production in Catharanthus roseus cell cultures, Plant Cell Reports, 1:139–142.
Di Cosmo, F., and Misawa, F., 1985, Eliciting secondary metabolism in plant cell cultures, Trends in Biotechnology, 3(12):318–322.
Deliu, C., Ispas, G., and Munteanu-Deliu, C., 1994, Methods for immobilization of plant cell cultures producing secondary metabolites, Studia Universitatis Babes-Bolyai Biologia, 39(1):59–68.
Dornenburg, H., and Knorr, D., 1995, Strategies for the improvement of secondary metabolite production in plant cell cultures, Enzyme and Microbial Technology, 17:674–684.
Farmer, W., and Liao, J., 1996, Progress in metabolic engineering, Current Opinion in Biotechnology, 7:198–204.
Fowke, L., Gresshoff, P., and Marchant, H., 1979, Transfer of organelles of the alga Chlamydomonas reinhardii into carrot cells by protoplast fusion, Planta, 144:341–347.
Fowke, L., Marchant, H., and Gresshoff, P., 1981, Fusion of protoplasts from carrot cell cultures and the green alga Stigeoclonium, Canadian Journal of Botany, 59:1021–1025.
Gundlach, H., Muller, M., Kutchan, T., and Zenk, M., 1992, Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures, Proceedings of the National Academy of Sciences, 89:2389–2393.
Hua, J., Erickson, L., Yiin, T., and Glasgow, L., 1993, A review of the effects of shear and interfacial phenomena on cell viability, Critical Reviews in Biotechnology, 13(4):305–328.
Jones, A., 1994, Surprising signals in plant cells, Science, 263:183–184.
Ketchum, R., Gibson, D., Croteau, R., and Shuler, M., 1998, The response of suspension cell cultures of Taxus following elicitation with methyl jasmonate, submitted.
Kutchan, T., 1995, Alkaloid biosynthesis—the basis for metabolic engineering of medicinal plants, The Plant Cell, 7:1059–1070.
Mirjalili, N., and Linden, J., 1995, Gas phase composition effects on suspension cultures of Taxus cuspidata, Biotechnology and Bioengineering, 48:123–132.
Mirjalili, N., and Linden, J., 1996, Methyl jasmonate induced production of Taxol in suspension cultures of Taxus cuspidata: ethylene interaction and induction models, Biotechnology Progress, 12(1):110–118.
Namdev, P., and Dunlop, E., 1995, Shear sensitivity of plant cells in suspensions, Applied Biochemistry and Biotechnology, 54:109–131.
Nishi, A., 1993, Elicitor-induced production of secondary metabolites in higher plants, Yakugaku Zasshi, 113(12):847–860.
O’Keefe, B., Mahady, G., Gills, J., and Beecher, C., 1997, Stable vindoline production in transformed cell cultures of Catharanthus roseus, Journal of Natural Products, 60:261–254.
Plaskett, L., 1979, The generation of hydrogen gas using the photosynthetic mechanism of green plants and solar energy (biophotolysis), ETSU Publication CR/20, March.
Roberts, S., and Shuler, M., 1997, Large-scale plant cell culture, Current Opinion in Biotechnology, 8:154–159.
Schlatmann, J., Nuutila, A., Van Gulik, W., ten Hoopen, H., Verpoorte, R, and Heijnen, J., 1993, Scale-up of ajmalicine production by plant cell cultures of Catharanthus roseus, Biotechnology and Bioengineering, 41:253–262.
Seo, S., Sano, H., and Ohashi, Y., 1997, Jasmonic acid in wound signal transduction pathways, Physiologia Plantarum, 101(4):740–745.
Stephanopoulos, G., and Sinskey, A., 1993, Metabolic engineering—methodologies and future prospects, Trends in Biotechnology, 11:392–396.
Stephanopoulos, G., 1994, Metabolic engineering, Current Opinion in Biotechnology, 5:196–200.
Sun, Y., 1997, M.S. thesis, Cornell University.
Tabata, M., and Fujita, Y., 1985, Production of shikonin by plant cell cultures, in Biotechnology in Plant Science (Zaitlin, M., ed.).
ten Hoopen, H., Gulik, W., Schlatmann, J., Moreno, P., Vinke, J., Heijnen, J., and Verpoorte, R., 1994, Ajmalicine production by cell cultures of Catharanthus roseus: from shake flask to bioreactor, Plant Cell, Tissue and Organ Culture, 38:85–91.
Tyler, R., Kurz, W., Paiva, N., and Chavadej, S., 1995, Bioreactors for surface-immobilized cells, Plant Cell, Tissue and Organ Culture, 42:81–90.
Yoshikawa, M., Yamaoka, N., and Takeuchi, Y., 1993, Elicitors: their significance and primary modes of action in the induction of plant defense reactions, Plant Cell Physiology, 34(8):1193–1173.
Yukimune, Y., Tabata, H., Higashi, Y., and Hara, Y., 1997, Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures, Nature Biotechnology, 14:1129–1132.
Zhong, J., Yu, J., and Yoshida, T., 1995, Recent advances in plant cell cultures in bioreactors, World Journal of Microbiology and Biotechnology, 11:461–467.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Roberts, S.C., Shuler, M.L. (1998). Strategies for Bioproduct Optimization in Plant Cell Tissue Cultures. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_59
Download citation
DOI: https://doi.org/10.1007/978-0-585-35132-2_59
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46057-9
Online ISBN: 978-0-585-35132-2
eBook Packages: Springer Book Archive