Abstract
In this section, we will deal with the nutrient mixtures that are usually called media. In actual use, these will almost always be supplemented with serum or another complex biological fluid (milk, embryo extracts, and plasma are examples), or, as discussed in Chapter 8, with a defined mixture of hormones and growth factors. The ongoing experimental work of replacing the complex mixtures with defined components, both nutrients and proteins, largely has been responsible both for our understanding of what the medium does in cell culture and for our increased technical ability to maintain a broad range of functional cells in vitro.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Biggers, J. D., Gwatrikin, R. B. L., and Heynes, S., 1961, Growth of embryonic avian and mammalian tibiae on a relatively simple chemically defined medium, Exp. Cell Res. 25:41–58.
Bottenstein, J., Hayashi, I., Hutchings, S. H., Masui, H., Mather, J., McClure, D. B., Okasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R., 1979, The growth of cells in serum free hormone supplemented media, Methods Enzymol. 58:94–109.
Brewer, G., Torricelli, J., Evege, E., and Price, P. J., 1994, Neurobasal super (TM) medium/B27 supplement: A new serum-free medium combination for survival of neurons, Focus 16:6–8.
Dulbecco, R., and Freeman, G., 1959, Plaque Production by the Polyoma Virus, Virology 8:396–405.
Eagle, H., 1955, Nutrition needs of mammalian cells in tissue culture, Science 122:501–504.
Eagle, H., 1959, Amino acid metabolism in mammalian cell cultures, Science 130:432–437.
Eagle, H., 1965, Propagation in a fluid medium of a human epidermoid carcinoma strain KB, Proc. Exp. Biol. Med. 89:362–364.
Evans, V S., Bryant, J. C., Kerr, H., and Schilling, E., 1964, Chemically defined media for cultivation of long-term cell strains from mammalian species, Exp. Cell Res. 36:439–448.
Evans, V. S., Bryant, J. C., Fioramonti, M. C., McQuilkin, W. T., Sanford, K. K., and Earle, W. R., 1956, Studies of nutrient media for tissue cells in vitro. I. A protein free chemically defined medium for cultivation of strain Leydig cells, Cancer Res. 16:77–86.
Fischer, G. A., and Sartorelli, A. C., 1964, Development, Maintenance and Assay of Drug Resistance, in: Eisen, H. N. (ed.), Methods in Medical Research. Vol. 10, Year Book Medical Publishers, Chicago, pp. 247–262.
Ham, R., 1965, Clonal growth of mammalian cells in a chemically defined synthetic medium, Proc. Natl. Acad. Sci. USA 53:288–293.
Ham, R. G., 1963, An improved nutrient solution for diploid Chinese hamster and human cell lines, Exp. Cell Res. 29:515–526.
Ham, R. G., and McKeehan, W. L. (eds.), 1979, Media and growth requirements, Methods Enzymol. 58:44–93.
Hamilton, W., and Ham, R., 1977, Clonal growth of Chinese hamster cell lines in protein-free media, In Vitro 13:537–547.
Knedler, A., and Ham, R. G., 1987, Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell. Dev. Biol. 23:481–491.
Leibovitz, A., 1963, The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere, Am. J. Hyg. 78:173–180.
Macpherson, I. A., and Stoker, M. 1962, Polyoma transformation of hamster cell clones—An investigation of genetic factors affecting cell competence, Virology 16:147–151.
Mather, J., Kaczarowski, E., Gabler, R., and Wilkins, F., 1986, Effects of water purity and additional of common water contaminants on the growth of cells in serum-free media, BioTechniques 4:56–63.
Mather, J. P., Saez, J. M., Dray, F., and Haour, F., 1983, Vitamin E prolongs survival and function of porcine Leydig cells in culture, Ada Endocrinol. 102:470–475.
Mather, J. P., and Sato, G. H., 1979, The growth of mouse melanoma cells in hormone-supplemented, serum-free medium, Exp. Cell Res. 120:191–200.
McCoy, T. A., Maxwell, M., and Kruse, P. E., 1959, Amino acid requirements of the Novikoff hepatoma in vitro, Proc. Soc. Exp. Biol. Med. 100:115–118.
McKeehan, W., Hamilton, W., and Ham, R., 1976, Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts, Proc. Natl. Acad. Sci. USA 73:2023–2027.
McKeehan, W. L., McKeehan, K. A., Hammond, S. L., and Ham, R. G., 1977. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro 13:399–416.
Moore, G. E., and Kitamura, H., 1968, Cell line derived from patient with myeloma, NY State J. Med. 68:2054–2060.
Morgan, J. R., Morton, H. J., and Parker, R. C., 1950, Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium, Proc. Soc. Exp. Biol. Med. 73:1–8.
Parker, R., Castor, L. N., and McCulloch, E. A., 1957, Altered cell strains in continuous culture, NY Acad. Sci. 5:303–313.
Perez-Infante, V., Bardin, C. W., Gunsalus, G. L., Musto, N. A., Rich, K. A., and Mather, J. P., 1986, Differential regulation of testicular transferrin and androgen-binding protein secretion in primary cultures of rat Sertoli cells, Endocrinology 118:383–392.
Roberts, P. E., Phillips, D. M., and Mather, J. M., 1990, Properties of a novel epithelial cell from immature rat lung: Establishment and maintenance of the differentiated phenotype, Am. J. Physiol. Lung Cell. Mol. Physiol 3:415–425.
Waymouth, C., 1959, Rapid proliferation of sublines of NCTC Clone 929 (Strain L) mouse cells in a simple chemically defined medium (MB 752/1), J. Natl. Cancer Inst. 22:1003–1015.
Williams, G. M., and Gunn, J. M., 1974, Long-term cell culture of adult rat liver epithelial cells, Exp. Cell Res. 89:139–142.
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
(1998). Media. In: Introduction to Cell and Tissue Culture. Introductory Cell and Molecular Biology Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-27571-0_4
Download citation
DOI: https://doi.org/10.1007/978-0-585-27571-0_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-45859-0
Online ISBN: 978-0-585-27571-0
eBook Packages: Springer Book Archive