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Handbook of Single-Molecule Biophysics pp 19–42Cite as

Single-Molecule Analysis of Biomembranes

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Abstract

Biomembranes are more than just a cell’s envelope – as the interface to the surrounding of a cell they carry key signalling functions. Consequentially, membranes are highly complex organelles: they host about thousand different types of lipids and about half of the proteome, whose interaction has to be orchestrated appropriately for the various signalling purposes. In particular, knowledge on the nanoscopic organization of the plasma membrane appears critical for understanding the regulation of interactions between membrane proteins. The high localization precision of ∼20 nm combined with a high time resolution of ∼1 ms made single molecule tracking an excellent technology to obtain insights into membrane nanostructures, even in a live cell context. In this chapter, we will highlight concepts to achieve superresolution by single molecule imaging, summarize tools for data analysis, and review applications on artificial and live cell membranes.

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Authors and Affiliations

  1. Physics of Life Processes, Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, 2333 CA Leiden, The Netherlands

    Thomas Schmidt

  2. Biophysics Institute, Johannes Kepler University Linz, Altenbergerstr. 69, A-4040 Linz, Austria

    Gerhard J. Schütz

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  1. Institut für Biophysik, Universität Linz, Altenberger Straße 69, Linz, 4040, Austria

    Peter Hinterdorfer

  2. Harvard Medical School, Harvard University, Longwood Ave. 250, Boston, 02115, U.S.A.

    Antoine Oijen

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Schmidt, T., Schütz, G.J. (2009). Single-Molecule Analysis of Biomembranes. In: Hinterdorfer, P., Oijen, A. (eds) Handbook of Single-Molecule Biophysics. Springer, New York, NY. https://doi.org/10.1007/978-0-387-76497-9_2

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