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Chromosome Microdissection and Utilization of Microisolated DNA

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Plant Cytogenetics

Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 4))

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Abstract

Plant chromosome microdissection techniques, together with the isolation and amplification methods of microisolated DNA, are described; and a number of applications (e.g., generation of chromosome-specific markers, physical mapping, cloning of chromosome-specific genes) of microisolated chromosomes are discussed.

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Abbreviations

BAC:

Bacterial artificial chromosomes

DOP-PCR:

Degenerate oligonucleotide-primed PCR

LA-PCR:

Linker adapter PCR

MDA:

Multiple-displacement amplification

rDNA:

Ribosomal DNA

YAC:

Yeast artificial chromosomes

References

  • Albani D, Cote MJ, Armstrong KC, Chen QF, Segal A, Robert LS (1993) PCR amplification of microdissected wheat chromosome arms in a simple single tube reaction. Plant J 4:899–903

    Article  CAS  PubMed  Google Scholar 

  • Busch W, Martin R, Herrmann RG, Hohmann U (1995) Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley (Hordeum vulgare L.). Genome 38:1082–1090

    Article  CAS  PubMed  Google Scholar 

  • Chen QF, Armstrong K (1995) Characterization of a library from single microdissected oat (Avena sativa L.) chromosome. Genome 38:706–714

    Article  CAS  PubMed  Google Scholar 

  • Cheng YM, Lin BY (2003) Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164:299–310

    CAS  PubMed  Google Scholar 

  • Dang BY, Hu ZM, Zhou YH, Cui LH, Wang LL, Li LC, Chen ZH (1998) Construction of single-chromosome DNA library from Lilium regale Wilson. Chin Sci Bull 43:434–439

    Article  CAS  Google Scholar 

  • Delichère C, Veuskens J, Hernould M, Barbacar N, Mouras A, Negrutiu I, Monéger F (1999) SIY1, the first active gene cloned from a plant Y chromosome, encodes a WD-repeat protein. EMBO J 18:4169–4179

    Article  PubMed  Google Scholar 

  • Dolezel J, Kubalakova M, Bartos J, Macas J (2004) Flow cytogenetics and plant genome mapping. Chromosome Res 12:77–91

    Article  CAS  PubMed  Google Scholar 

  • Donald TM, Houben A, Leach CR, Timmis JN (1997) Ribosomal RNA genes specific to the B chromosomes in Brachycome dichromosomatica are not transcribed in leaf tissue. Genome 40:674–681

    Article  CAS  PubMed  Google Scholar 

  • Dong YZ, Liu ZL, Liu B, Bu XL, He MY, Huang BQ, Hao S (2002) Microdissection of individual chromosomes of Thinopyrum intermedium and isolation of molecular markers that are useful in detecting Th. intermedium chromatin introgressed into wheat. Cereal Res Commun 30:253–260

    CAS  Google Scholar 

  • Fuchs J, Houben A, Brandes A, Schubert I (1996a) Chromosome “painting” in plants – a feasible technique? Chromosoma 104:315–320

    CAS  PubMed  Google Scholar 

  • Fuchs J, Kloos DU, Ganal MW, Schubert I (1996b) In situ localization of yeast artificial chromosome sequences on tomato and potato metaphase chromosomes. Chromosome Res 4:277–281

    Article  CAS  PubMed  Google Scholar 

  • Fukui K, Minezawa M, Kamisugi Y, Ishikawa M, Ohmido N, Yanagisawa T, Fujishita M, Sakai F (1992) Microdissection of plant chromosomes by argon-ion laser beam. Theor Appl Genet 84:787–791

    CAS  PubMed  Google Scholar 

  • Gill KS, Gill BS, Endo TR, Boyko EV (1996) Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics 143:1001–1012

    CAS  PubMed  Google Scholar 

  • Grant S, Houben A, Vyskot B, Siroky J, Pan WH, Macas J, Saedler H (1994) Genetics of sex determination in flowering plants. Dev Genet 15:214–230

    Article  Google Scholar 

  • Gribble S, Ng BL, Prigmore E, Burford DC, Carter NP (2004) Chromosome paints from single copies of chromosomes. Chromosome Res 12:143–151

    Article  CAS  PubMed  Google Scholar 

  • Hagag NG, Viola MV (1993) Chromosome Microdissection and Cloning: A Practical Guide. Academic Press, San Diego

    Google Scholar 

  • Hellani A, Coskun S, Benkhalifa M, Tbakhi A, Sakati N, Al-Odaib A, Ozand P (2004) Multiple displacement amplification on single cell and possible PGD applications. Mol Hum Reprod 10:847–852

    Article  CAS  PubMed  Google Scholar 

  • Hernould M, Glimelius K, Veuskens J, Bergman P, Mouras A (1997) Microdissection and amplification of coding sequences from a chromosome fragment restoring male fertility in alloplasmic male-sterile tobacco. Plant J 12:703–709

    Article  CAS  PubMed  Google Scholar 

  • Hizume M, Shibata F, Maruyama Y, Kondo T (2001) Cloning of DNA sequences localized on proximal fluorescent chromosome bands by microdissection in Pinus densiflora Sieb. & Zucc. Chromosoma 110:345–351

    Article  CAS  PubMed  Google Scholar 

  • Hobza R, Vyskot B (2007) Laser micro dissection-based analysis of plant sex chromosomes. Laser Manip Cells Tissues 82:433–453

    Article  CAS  Google Scholar 

  • Hobza R, Lengerova M, Cernohorska H, Rubes J, Vyskot B (2004) FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia. Chromosome Res 12:245–250

    Article  CAS  PubMed  Google Scholar 

  • Hosono S, Faruqi AF, Dean FB, Du Y, Sun Z, Wu X, Du J, Kingsmore SF, Egholm M, Lasken RS (2003) Unbiased whole-genome amplification directly from clinical samples. Genome Res 13:954–964

    Article  CAS  PubMed  Google Scholar 

  • Houben A, Franke J, Leclerc N, Ahne R (1996a) Computer-assisted system combining image analysis and chromosome dissection. Microsc Res Tech 34:474–477

    Article  CAS  PubMed  Google Scholar 

  • Houben A, Kynast RG, Heim U, Hermann H, Jones RN, Forster JW (1996b) Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale). Chromosoma 105:97–103

    Article  CAS  PubMed  Google Scholar 

  • Houben A, Leach CR, Verlin D, Rofe R, Timmis JN (1997) A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome. Chromosoma 106:513–519

    CAS  PubMed  Google Scholar 

  • Hu ZM, Wang H, Shi R, Dang BY, Hu J, Yin WB, Chen YH, Jung SM, Chen ZH (2004) Microdissection and construction of region-specific DNA libraries of wheat chromosome 6B. Acta Bot Sin 46:1357–1365

    CAS  Google Scholar 

  • Huang D, Wu W, Zhou Y, Hu Z, Lu L (2004) Microdissection and molecular manipulation of single chromosomes in woody fruit trees with small chromosomes using pomelo (Citrus grandis) as a model. I. Construction of single chromosomal DNA libraries. Theor Appl Genet 108:1366–1370

    Article  CAS  PubMed  Google Scholar 

  • Jamilena M, Garrido-Ramos M, Ruiz Rejón M, Ruiz Rejón C, Parker JS (1995) Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris. Chromosoma 104:113–120

    Article  CAS  PubMed  Google Scholar 

  • Jiang JM, Gill BS, Wang GL, Ronald PC, Ward DC (1995) Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes. Proc NatlAcad Sci USA 92:4487–4491

    Article  CAS  Google Scholar 

  • Jung C, Claussen U, Horsthemke B, Fischer F, Herrmann RG (1992) A DNA library from an individual Beta patellaris chromosome conferring nematode resistance obtained by microdissection of meiotic metaphase chromosomes. Plant Mol Biol 20:503–511

    Article  CAS  PubMed  Google Scholar 

  • Kejnovsky E, Hobza R, Kubat Z, Widmer A, Marais GAB, Vyskot B (2007) High intrachromosomal similarity of retrotransposon long terminal repeats: evidence for homogenization by gene conversion on plant sex chromosomes? Gene 390:92–97

    Article  CAS  PubMed  Google Scholar 

  • Kunzel G, Korzun L, Meister A (2000) Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints. Genetics 154:397–412

    CAS  PubMed  Google Scholar 

  • Leach CR, Houben A, Field B, Pistrick K, Demidov D, Timmis JN (2005) Molecular evidence for transcription of genes on a B chromosome in Crepis capillaris. Genetics 171:269–278

    Article  CAS  PubMed  Google Scholar 

  • Liu B, Segal G, Vega JM, Feldman M, Abbo S (1997) Isolation and characterization of chromosome-specific DNA sequences from a chromosome arm genomic library of common wheat. Plant J 11:959–965

    Article  CAS  Google Scholar 

  • Ludecke HJ, Senger G, Claussen U, Horsthemke B (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature 338:348–350

    Article  CAS  PubMed  Google Scholar 

  • Lysak MA, Fransz PF, Ali HBM, Schubert I (2001) Chromosome painting in Arabidopsis thaliana. Plant J 28:689–697

    Article  CAS  PubMed  Google Scholar 

  • Macas J, Weschke W, Bumlein H, Pich U, Houben A, Wobus U, Schubert I (1993) Localization of vicilin genes via polymerase chain reaction on microisolated field bean chromosomes. Plant J 3:883–886

    Article  CAS  PubMed  Google Scholar 

  • Mariotti B, Navajas-Perez R, Lozano R, Parker JS, de la Herran R, Ruiz Rejón C, Ruiz Rejón M, Garrido-Ramos M, Jamilena M (2006) Cloning and characterization of dispersed repetitive DNA derived from microdissected sex chromosomes of Rumex acetosa. Genome 49:114–121

    CAS  PubMed  Google Scholar 

  • Marschner S, Meister A, Blattner FR, Houben A (2007) Evolution and function of B chromosome 45S rDNA sequences in Brachycome dichromosomatica. Genome 50:638–644

    Article  CAS  PubMed  Google Scholar 

  • Matsunaga S, Kawano S, Michimoto T, Higashiyama T, Nakao S, Sakai A, Kuroiwa T (1999) Semi-automatic laser beam microdissection of the Y chromosome and analysis of Y chromosome DNA in a dioecious plant, Silene latifolia. Plant Cell Physiol 40:60–68

    Article  CAS  PubMed  Google Scholar 

  • Meltzer PS, Guan XY, Burgess A, Trent JM (1992) Rapid generation of region specific probes by chromosome microdissection and their application. Nat Genet 1:24–28

    Article  CAS  PubMed  Google Scholar 

  • Meltzer PS, Guan XY, Su YA, Gracia E, Trent JM (1997) Identification of region specific genes by chromosome microdissection. Cancer Genet Cytogenet 93:29–32

    Article  CAS  PubMed  Google Scholar 

  • Pich U, Houben A, Fuchs J, Meister A, Schubert I (1994) Utility of DNA amplified by degenerate oligonucleotide-primed PCR (DOP-PCR) from the total genome and defined chromosomal regions of field bean. Mol Gen Genet 243:173–177

    CAS  PubMed  Google Scholar 

  • Potz H, Schubert V, Houben A, Schubert I, Weber WE (1996) Aneuploids as a key for new molecular cloning strategies: development of DNA markers by microdissection using Triticum aestivum–Aegilops markgrafii chromosome addition line B. Euphytica 89:41–47

    Article  CAS  Google Scholar 

  • Sandery MJ, Forster JW, Macadam SR, Blunden R, Jones RN, Brown DM (1991) Isolation of a sequence common to A- and B-chromosomes of rye (Secale cereale) by microcloning. Plant Mol Biol Rep 9:21–30

    Article  CAS  Google Scholar 

  • Schondelmaier J, Martin R, Jahoor A, Houben A, Graner A, Koop HU, Herrmann RG, Jung C (1993) Microdissection and microcloning of the barley (Hordeum vulgare L.) chromosome 1Hs. Theor Appl Genet 86:629–636

    Article  CAS  PubMed  Google Scholar 

  • Shibata F, Hizume M (2002) Evolution of 5S rDNA units and their chromosomal localization in Allium cepa and Allium schoenoprasum revealed by microdissection and FISH. Theor Appl Genet 105:167–172

    Article  CAS  PubMed  Google Scholar 

  • Shibata F, Hizume M, Kuroki Y (1999) Chromosome painting of Y chromosomes and isolation of a Y chromosome-specific repetitive sequence in the dioecious plant Rumex acetosa. Chromosoma 108:266–270

    Article  CAS  PubMed  Google Scholar 

  • Sorokin A, Marthe F, Houben A, Pich U, Graner A, Kunzel G (1994) Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley. Genome 37:550–555

    Article  CAS  PubMed  Google Scholar 

  • Stein N, Ponelies N, Musket T, McMullen M, Weber G (1998) Chromosome micro-dissection and region-specific libraries from pachytene chromosomes of maize (Zea mays L.). Plant J 13:281–289

    Article  CAS  Google Scholar 

  • Telenius H, Carter NP, Bebb CE, Nordenskjold M, Ponder BAJ, Tunnacliffe A. (1992) Degenerate oligonucleotide-primed PCR – general amplification of target DNA by a single degenerate primer. Genomics 13:718–725

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Zhou YH, Dang BY, Hu ZM, Wang LL, Li LC, Chen ZH (1998) Chromosome microdissection by laser microbeam, chromosomal fragment isolation and amplification in vitro in barley (Hordeum vulgare L.). Chin Sci Bull 43:851–855

    Article  CAS  Google Scholar 

  • Zhang K, Martiny AC, Reppas NB, Barry KW, Malek J, Chisholm SW, Church GM (2006) Sequencing genomes from single cells by polymerase cloning. Nat Biotechnol 24:680–686

    Article  CAS  PubMed  Google Scholar 

  • Zhou RN, Hu ZM (2007) The development of chromosome microdissection and microcloning technique and its applications in genomic research. Curr Genomics 8:67–72

    Article  CAS  PubMed  Google Scholar 

  • Zhou RN, Shi R, Jiang SM, Yin WB, Wang HH, Chen YH, Hu J, Wang RR, Zhang XQ, Hu ZM (2008) Rapid EST isolation from chromosome 1R of rye. BMC Plant Biol 8:28

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhou YH, Hu ZM, Dang BY, Wang HA, Deng XD, Wang LL, Chen ZH (1999) Microdissection and microcloning of rye (Secale cereale L.) chromosome 1R. Chromosoma 108:250–255

    Article  CAS  PubMed  Google Scholar 

  • Zhou YH, Dang BY, Wang H, Hu ZM, Wang LL, Chen ZH (2001) Microdissection of a single chromosome and construction of the microclone library from soybean. Euphytica 121:129–135

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Chromosome Structure and Function Laboratory, Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Saxony-Anhalt, Germany

    Andreas Houben

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  1. Andreas Houben
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Correspondence to Andreas Houben .

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Editors and Affiliations

  1. Department of Biological Science, Florida State University, Tallahassee, Florida, USA

    Hank W. Bass

  2. Division of Biological Sciences, University of Missouri, Columbia, Missouri, USA

    James A. Birchler

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Houben, A. (2012). Chromosome Microdissection and Utilization of Microisolated DNA. In: Bass, H., Birchler, J. (eds) Plant Cytogenetics. Plant Genetics and Genomics: Crops and Models, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-0-387-70869-0_10

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