Abstract
Purpose: To investigate the safety range of current by suprachoroidal-transretinal stimulation (STS) using a high-current continuous stimulation. Method: Sclerotomy was performed at the area just beneath the visual streak of rabbits and the platinum (Pt) electrode (diameter: 100μm or 200μm) embedded in silicone plate was attached on the fenestrated sclera. Return electrode was placed in the vitreous cavity. Retina was stimulated by biphasic pulses (anodic first, duration: 0.5,msec, frequency: 20,Hz) with a current ranged from 1 to 3,mA continuously for an hour. The rabbit eyes were enucleated immediately after microscopic fundus observation, fixated with glutar-aldehide, embedded in paraffin and stained with hematoxylin-eosin.
Result: For 100μm electrode, no histological change was observed with a current of 1 mA, but retinal change was observed with a current of 1.5 mA.
For 200 μm electrode, no histological change was observed with a current of 1.5 mA but with a current of 2.0 mA, retinal change was observed. The residual scleral thickness was 50–100 μm.
Conclusion: The results of acute experiment suggested that a relatively large amount of current was able to be injected with STS method without tissue damages. In the next step, an experiment with chronic stimulation is needed to verify the safety of STS method.
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Nakauchi, K., Fujikado, T., Hirakata, A., Tano, Y. (2007). A Tissue Change After Suprachoroidal-Transretinal Stimulation with High Electrical Current in Rabbits. In: Humayun, M.S., Weiland, J.D., Chader, G., Greenbaum, E. (eds) Artificial Sight. Biological and Medical Physics, Biomedical Engineering. Springer, New York, NY. https://doi.org/10.1007/978-0-387-49331-2_17
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