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Measuring the Real Point Spread Function of High Numerical Aperture Microscope Objective Lenses

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Handbook Of Biological Confocal Microscopy

Abstract

Testing and characterization of high-quality lenses have been perfected into fine art with the advent of lasers, phase-shifting interferometers, charge-coupled device (CCD) cameras, and computers. A bewildering array of techniques is described in Malacara’s classic reference book on the subject (Malacara, 1992). Several of these techniques, in particular the Twyman–Green interferometer and the star test, are applicable to testing of microscope objective lens.

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Juškaitis, R. (2006). Measuring the Real Point Spread Function of High Numerical Aperture Microscope Objective Lenses. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_11

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