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Root Hair Electrophysiology

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Plant Cell Monographs

Part of the book series: Plant Cell Monographs

Abstract

In this review, the value of root hairs as a model system for a broad range of electrophysiological experiments is highlighted by an introductory case study of their use for in situ measurements of vacuolar electrophysiology. Then, the basic tenets of electrophysiological measurements are presented: the electrical properties of the cell and how they are measured. Recent research on ion transporters (pumps, channels, and coupled transporters) is reviewed, followed by a discussion of the role of ion transport in root hair morphogenesis.

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References

  • Ahn SJ, Shin R, Schachtman DP (2004) Expression of KT/KUPgenes in Arabidopsisand the role of root hairs in K+uptake. Plant Physiol 134:1135–1145

    Article  PubMed  CAS  Google Scholar 

  • Ammann D (1986) Ion-selective microelectrodes. Principles, design and application. Springer, Berlin Heidelberg New York, pp 1–346

    Google Scholar 

  • Arango M, Gevaudant F, Oufattole M, Boutry M (2003) The plasma membrane proton pump ATPase: The significance of gene subfamilies. Planta 216:355–365

    PubMed  CAS  Google Scholar 

  • Ashley MK, Grant M, Grabov A (2006) Plant responses to potassium deficiencies: a role for potassium transport proteins. J Exp Bot 57:425–436

    Article  PubMed  CAS  Google Scholar 

  • Ayling SM (1993) The effect of ammonium ions on membrane potential and anion flux in roots of barley and tomato. Plant Cell Environ 16:297–303

    Article  CAS  Google Scholar 

  • Ayling SM, Brownlee C, Clarkson DT (1994) The cytoplasmic streaming response of tomato root hairs to auxin: observations of cytosolic calcium levels. J Plant Physiol 143:184–188

    CAS  Google Scholar 

  • Bates TR, Lynch JP (1996) Stimulation of root hair elongation in Arabidopsis thalianaby low phosphorus availability. Plant Cell Environ 19:529–538

    Article  CAS  Google Scholar 

  • Blatt MR (1991) A primer in plant electrophysiological methods. In: Hostettmann K (ed) Methods in plant biochemistry, vol 6. Assays for bioactivity. Academic, London (xi and 360 pp), pp 281–321 (ISBN: 0124610161)

    Google Scholar 

  • Bouteau F, Pennarun A-M, Kurkdjian A, Convert M, Cornel D, Monestiez M, Rona J-P, Bousquet U (1999) Ion channels of intact young root hairs from Medicago sativa. Plant Physiol Biochem 37:889–898

    Article  CAS  Google Scholar 

  • Briskin DP, Gawienowski MC (1996) Role of the plasma membrane H+-ATPase in K+transport. Plant Physiol 111:1199–1207

    PubMed  CAS  Google Scholar 

  • Campanoni P, Sutter JU, Davis CS, Littlejohn GR, Blatt MR (2007) A generalized method for transfecting root epidermis uncovers endosomal dynamics in Arabidopsisroot hairs. Plant J 51:322–330

    Article  PubMed  CAS  Google Scholar 

  • Daram P, Brunner S, Persson BL, Amrhein N, Bucher M (1998) Functional analysis and cell-specific expression of a phosphate transporter from tomato. Planta 206:225–233

    Article  PubMed  CAS  Google Scholar 

  • Desbrosses G, Josefsson C, Rigas S, Hatzopoulos O, Dolan L (2003) AKT1and TRH1are required during root hair elongation in Arabidopsis. J Exp Bot 54:781–788

    Article  PubMed  CAS  Google Scholar 

  • Diatloff E, Roberts M, Sanders D, Roberts SK (2004) Characterization of anion channels in the plasma membrane of Arabidopsisepidermal root cells and the identification of a citrate-permeable channel induced by phosphate starvation. Plant Physiol 136:4136–4149

    Article  PubMed  CAS  Google Scholar 

  • Dutta R, Robinson KR (2004)[B1] Identification and characterization of stretch-activated ion channels in pollen protoplasts. Plant Physiol 135:1398–1406

    Article  CAS  Google Scholar 

  • Etherton B, Keifer DW, Spanswick RM (1977) Comparison of three methods for measuring electrical resistances of plant cell membranes. Plant Physiol 60:684–688

    PubMed  CAS  Google Scholar 

  • Felle H (1982) Effects of fusicoccin upon membrane potential, resistance and current-voltage characteristics in root hairs of Sinapis alba. Plant Sci Lett 25:219–225

    Article  CAS  Google Scholar 

  • Felle HH (1993) Ion-selective microelectrodes: their use and importance in modern cell biology. Bot Acta 106:5–12

    CAS  Google Scholar 

  • Felle HH (1994) The H+/Cl−symporter in root hairs of Sinapis alba: an electrophysiolgoical study using ion-selective microelectrodes. Plant Physiol 106:1131–1136

    PubMed  CAS  Google Scholar 

  • Felle HH (1996) Control of cytoplasmic pH under anoxic conditions and its implication for plasma membrane proton transport in Medicago sativaroot hairs. J Exp Bot 47:967–973

    Article  CAS  Google Scholar 

  • Felle H, Hepler PK (1997) The cytosolic Ca2+concentration gradient of Sinapsis albaroot hairs as revealed by Ca2+-selective microelectrode tests and fura-dextran ratio imaging. Plant Physiol 114:39–45

    PubMed  CAS  Google Scholar 

  • Felle HH, Herrmann A (2000) pH regulation in and by root hairs. In: Ridge RW, Emons AMC (eds) Root hairs. Cell and molecular biology. Springer, Berlin Heidelberg New York, pp 165–178

    Google Scholar 

  • Felle HH, Kondorosi É, Kondorosi Á, Schultze M (1998) The role of ion fluxes in Nod factor signalling in Medicago sativa. Plant J 13:455–463

    Article  CAS  Google Scholar 

  • Finkel AS, Redman S (1984) Theory and operation of a single microelectrode voltage clamp. J Neurosci Meth 11:101–127

    Article  CAS  Google Scholar 

  • Fox TC, Guerinot ML (1998) Molecular biology of cation transport in plants. Annu Rev Plant Physiol Plant Mol Biol 49:669–696

    Article  PubMed  CAS  Google Scholar 

  • Goedhart J, Gadella TWJ Jr (2000) Fluorescence microspectroscopic methods. In: Ridge RW, Emons AMC (eds) Root hairs. Cell and molecular biology. Springer, Berlin Heidelberg New York, pp 65–94

    Google Scholar 

  • Gassmann W, Schroeder JI (1994) Inward-rectifying K+channels in root hairs of wheat. A mechanism for aluminum-sensitive low-affinity K+uptake and membrane potential control. Plant Physiol 105:1399–1408

    PubMed  CAS  Google Scholar 

  • Grabov A, Bottger M (1994) Are redox reactions involved in regulation of K+channels in the plasma membrane of Limnobium stoloniferumroot hairs? Plant Physiol 105:927–935

    PubMed  CAS  Google Scholar 

  • Higgins R, Lockwood T, Holley S, Yalamanchili R, Stratmann JW (2006) Changes in extracellular pH are neither required nor sufficient for activation of mitogen-activated protein kinases (MAPKs) in response to systemin and fusicoccin in tomato. Planta doi 10.1007/s00425–006–0440–8

    Google Scholar 

  • Hille B (1984) Ionic channels of excitable membranes. Sinauer Associates, Sunderland, ix and 426 pp

    Google Scholar 

  • Hirsch RE, Lewis BD, Spalding EP, Sussman MR (1998) A role for the AKT1 potassium channel in plant nutrition. Science 280:918–921

    Article  PubMed  ADS  CAS  Google Scholar 

  • Hopkins L, Parmar S, Blaszczyk A, Hesse H, Hoefgen R, Hawkesford MJ (2005) O-Acetylserine and the regulation of expression of genes encoding components for sulfate uptake and assimilation in potato. Plant Physiol 138:433–440

    Article  PubMed  CAS  Google Scholar 

  • Ivashikina N, Becker D, Ache P, Meyerhoff O, Felle HH, Hedrich R (2001) K+channel profile and electrical properties of Arabidopsisroot hairs. FEBS Lett 508:463–469

    Article  PubMed  CAS  Google Scholar 

  • Jones DL, Shaff JE, Kochian LV (1995) Role of calcium and other ions in directing root hair tip growth in Limnobium stoloniferum. I. Inhibition of tip growth by aluminum. Planta 197:672–680

    Article  CAS  Google Scholar 

  • Jungk A (2001) Root hairs and the acquisition of plant nutrients from soil. J Plant Nutr Soil Sci 164:121–129

    Article  CAS  Google Scholar 

  • Kanamori N, Madsen LH, Radutoiu S, Frantescu M, Quistgaard EM, Miwa H, Downie JA, James EK, Felle HH, Haaning LL, Jensen TH, Sato S, Nakamura Y, Tabata S, Sandal N, Stougaard J (2006) A nucleoporin is required for induction of Ca2+spiking in legume nodule development and essential for rhizobial and fungal symbiosis. Proc Natl Acad Sci USA 103:359–364

    Article  PubMed  ADS  CAS  Google Scholar 

  • Kanczewska J, Marco S, Vandermeeren C, Maudoux O, Rigaud J-L, Boutry M (2005) Activation of the plant plasma membrane H+-ATPase by phosphorylation and binding of 14–3–3 proteins converts a dimer into a hexamer. Proc Natl Acad Sci USA 102:11675–11680

    Article  PubMed  ADS  CAS  Google Scholar 

  • Kojima S, Bohner A, Gassert B, Yuan L, von Wirén N (2007) AtDUR3 represents the major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsisroots. Plant J 52:30–40

    Article  PubMed  CAS  Google Scholar 

  • Konno M, Ooishi M, Inoue Y (2006) Temporal and positional relationships between Mn uptake and low-pH-induced root hair formation in Lactuca sativacv. Grand Rapids seedlings. J Plant Res 119:439–447

    Article  PubMed  CAS  Google Scholar 

  • Kopriva S (2006) Regulation of sulfate assimilation in Arabidopsisand beyond. Ann Bot 97:479–495

    Article  PubMed  CAS  Google Scholar 

  • Kurkdjian AC (1995) Role of the differentiation of root epidermal cells in Nod factor (from Rhizobium meliloti)-induced root-hair depolarization of Medicago sativa. Plant Physiol 107:783–790

    PubMed  CAS  Google Scholar 

  • Kurkdjian A, Bouteau F, Pennarun A-M, Convert M, Cornel D, Rona J-P, Bousquet U (2000) Ion currents involved in early Nod factor response in Medicago sativumroot hairs: a discontinuous single-electrode voltage-clamp study. Plant J 22:9–17

    Article  PubMed  CAS  Google Scholar 

  • Lauger P (1991) Electrogenic Ion Pumps. Sinauer Associates, Sunderland, xi + 313 pp.

    Google Scholar 

  • Lauter F-R, Ninnemann O, Bucher M, Riesmeier JW, Frommer WB (1996) Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato. Proc Natl Acad Sci USA 93:8139–8144

    Article  PubMed  ADS  CAS  Google Scholar 

  • Lefebvre B, Arango M, Oufattole M, Crouzet J, Purnelle B, Boutry M (2005) Identification of a Nicrotiana plumbaginifoliaplasma membrane H+-ATPase gene expressed in the pollen tube. Plant Mol Biol 58:775–787

    Article  PubMed  CAS  Google Scholar 

  • Legarde D, Basset M, Lepetit M, Conejero G, Gaymard F, Astruc S, Grignon C (1996) Tissue-specific expression of ArabidopsisAKT1 gene is consistent with a role in K+nutrition. Plant J 9:195–203

    Article  Google Scholar 

  • Lew RR (1989) Calcium activates an electrogenic proton pump in Neurosporaplasma membrane. Plant Physiol 91:213–216

    PubMed  CAS  Google Scholar 

  • Lew RR (1991) Electrogenic transport properties of growing Arabidopsis thalianaroot hairs. The plasma membrane proton pump and potassium channels. Plant Physiol 97:1527–1534

    PubMed  CAS  Google Scholar 

  • Lew RR (1994) Regulation of electrical coupling between Arabidopsisroot hairs. Planta 193:67–73

    Article  CAS  Google Scholar 

  • Lew RR (1996) Pressure regulation of the electrical properties of growing Arabidopsis thalianaL. root hairs. Plant Physiol 112:1089–1100

    Article  PubMed  ADS  CAS  Google Scholar 

  • Lew RR (1998a) Mapping fungal ion channel locations. Fung Genet Biol 24:69–76

    Article  CAS  Google Scholar 

  • Lew RR (1998b) Immediate and steady state extracellular ionic fluxes of growing Arabidopsis thaliana root hairs under hyperosmotic and hypoosmotic conditions. Physiol Plant 104:397–404

    Article  CAS  Google Scholar 

  • Lew RR (2000) Root hair electrobiology. In: Ridge RW, Emons AMC (eds) Root Hairs. cell and molecular biology. Springer, Berlin Heidelberg New York, pp 115–139

    Google Scholar 

  • Lew RR (2004) Osmotic effects on the electrical properties of Arabidopsis thalianaL. root hair vacuoles in situ. Plant Physiol 134:352–360

    Article  PubMed  CAS  Google Scholar 

  • Lew RR (2006) Use of double barrel micropipettes to voltage-clamp plant and fungal cells. In Volkov AG (ed) Plant electrophysiology. Theory and methods. Springer, Berlin Heidelberg New York, pp 139–154

    Google Scholar 

  • Lew RR, Levina NN, Shabala L, Anderca MI, Shabala SN (2006) Role of a MAP kinase cascade in ion flux-mediated turgor regulation in fungi. Eukaryotic Cell 5:480–487

    Article  PubMed  CAS  Google Scholar 

  • Li L, Kim B-G, Cheong YH, Pandey GK, Luan S (2006) A Ca2+signaling pathway regulates a K+channel for low-K response in Arabidopsis. Proc Natl Acad Sci USA 103:12625–12630

    Article  PubMed  ADS  CAS  Google Scholar 

  • Liu C, Muchhal US, Uthappa M, Kononowicz AK, Raghothama KG (1998) Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus. Plant Physiol 116:91–99

    Article  PubMed  CAS  Google Scholar 

  • Loque D, Yuan L, Kojima S, Gojon A, Wirth J, Gazzarrini S, Ishiyama K, Takahashi H, von Wiren N (2006) Additive contribution of AMT1;1 and AMT1:3 to high-affinity ammonium uptake across the plasma membrane of nitrogen-deficient Arabidopsisroots. Plant J 48:522–534

    Article  PubMed  CAS  Google Scholar 

  • Ludewig U, Wilken S, Wu B, Jost W, Obrdlik P, El Bakkoury M, Marini A-M, Andre B, Hamacher T, Boles E, von Wiren N, Frommer WB (2003) Homo- and hetero-oligomerization of ammonium transporter-1 uniporters. J Biol Chem 278:45603–45610

    Article  PubMed  CAS  Google Scholar 

  • Ma JF, Goto S, Tamai K, Ichii M (2001) Role of root hairs and lateral roots in silicon uptake by rice. Plant Physiol 127:1773–1780

    Article  PubMed  CAS  Google Scholar 

  • Maathuis FJM, Sanders D (1993) Energization of potassium uptake in Arabidopsis thaliana. Planta 191:302–307.

    Article  CAS  Google Scholar 

  • Maathuis FJM, Sanders D (1995) Contrasting roles of two K+-channel types in root cells of Arabidopsis thaliana. Planta 197:456–464

    Article  PubMed  CAS  Google Scholar 

  • MacRobbie EAC (2006) Osmotic effects on vacuolar ion release in guard cells. Proc Natl Acad Sci USA 103:1135–1140

    Article  PubMed  ADS  CAS  Google Scholar 

  • Maruyama-Nakashita A, Nakamura Y, Yamaya T, Takahashi H (2004) Regulation of high-affinity sulphate transporters in plants: towards a systematic analysis of sulphur signalling and regulation. J Exp Bot 55:1843–1849

    Article  PubMed  CAS  Google Scholar 

  • Maser P, Thomine S, Schroeder JI, Ward JM, Hirschi K, Sze H, Talke IN, Amtmann A, Maathuis FJM, Sanders D, Harper JF, Tchieu J, Gribskov M, Persans MW, Salt DE, Kim SA, Guerinot ML (2001) Phylogenetic relationships within cation transporter families of Arabidopsis. Plant Physiol 126:1646–1667

    Article  PubMed  CAS  Google Scholar 

  • Masucci JD, Schiefelbein JW (1994) The rhd6 mutation of Arabidopsis thalianaalters root-hair initiation through an auxin- and ethylene-associated process. Plant Physiol 106:1335–1346

    PubMed  CAS  Google Scholar 

  • Meharg AA, Blatt MR (1995) transport across the plasma membrane of Arabidopsis thalianaroot hairs: Kinetic control by pH and membrane voltage. J Memb Biol 145:49–66

    Article  CAS  Google Scholar 

  • Michelet B, Boutry M (1995) The plasma membrane H+-ATPase. A highly regulated enzyme with multiple physiological functions. Plant Physiol 108:1–6

    PubMed  CAS  Google Scholar 

  • Miller AJ, Wells DM (2006) Electrochemical methods and measuring transmembrane ion gradients. InVolkov AG (ed) Plant electrophysiology. Theory and methods. Springer, Berlin Heidelberg New York, pp 15–34

    Google Scholar 

  • Moog PR, van der Kooij TAW, Bruggemann W, Schielfelbein JW, Kuiper PJC (1995) Responses to iron deficiency in Arabidopsis thaliana: The Turbo iron reductase does not depend on the for-mation of root hairs and transfer cells. Planta 195:505–513

    Article  PubMed  CAS  Google Scholar 

  • Moriau L, Michelet B, Bogaerts P, Lambert L, Michel A, Oufattole M, Boutry M (1999) Expression analysis of two gene sub-families encoding the plasma membrane H+-ATPase in Nicotiana plumbaginifoliarevelas the major transport functions of this enzyme. Plant J 19:31–41

    Article  PubMed  CAS  Google Scholar 

  • Noble PS (1991) Physicochemical and environmental plant physiology. Academic, San Diego, xx and 635 pp

    Google Scholar 

  • Ogden D (1994) Microelectrode techniques. The Plymouth workshop handbook, 2nd edn. The Company of Biologists Ltd, Cambridge, x and 448 pp

    Google Scholar 

  • Palmgren MG (1998) Proton gradients and plant growth: role of the plasma membrane H+-ATPase. Adv Bot Res 28:1–70

    Article  CAS  Google Scholar 

  • Palmgren MG (2001) Plant plasma membrane H+-ATPases: powerhouses for nutrient uptake. Annu Rev Plant Physiol Plant Mol Biol 52:817–845

    Article  PubMed  CAS  Google Scholar 

  • Peterson RL, Stevens KJ (2000) Evidence for the uptake of non-essential ions and essential nutrient ions by root hairs and their effect on root hair development. In Ridge RW, Emons AMC (eds) Root hairs. Cell and molecular biology. Springer, Berlin Heidelberg New York, pp 179–195

    Google Scholar 

  • Purves RD (1981) Microelectrode methods of intracellular recording and ionophoresis. Academic, London, x and 146 pp

    Google Scholar 

  • Ramirez JA, Vacata V, McCusker JH, Haber JE, Mortimer RK, Owen WG, Lecar H (1989) ATP-sensitive K+channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci USA 86:7866–7870

    Article  PubMed  ADS  CAS  Google Scholar 

  • Reintanz B, Szyroki A, Ivashikina N, Ache P, Godde M, Becker D, Palme K, Hedrich R (2002) AtKC1, a silent Arabidopsispotassium channel alpha-subunit modulates root hair K+influx. Proc Natl Acad Sci USA 99:4079–4084

    Article  PubMed  ADS  CAS  Google Scholar 

  • Rigas S, Debrosses G, Haralampidis K, Vicente-Agullo F, Feldmann KA, Grabov A, Dolan L, Hatzopoulos P (2001) TRH1encodes a potassium transporter required for tip growth in Arabidopsisroot hairs. Plant Cell 13:139–151

    Article  PubMed  CAS  Google Scholar 

  • Roberts SK (2006) Plasma membrane anion channels in higher plants and their putative functions in roots. New Phytol 169:647–666

    Article  PubMed  Google Scholar 

  • Robinson NJ, Procter CM, Connolly EL, Guerinot ML (1999) A ferric-chelate reductase for iron uptake from soils. Nature 397:694–697

    Article  PubMed  ADS  CAS  Google Scholar 

  • Sakmann B, Neher E (1995) Single channel recording, 2nd edn. Plenum, New York, xxii and 700pp

    Google Scholar 

  • Sanchez-Fernandez R, Fricker M, Corben LB, White NS, Sheard N, Leaver CJ, Van Montagu M, Inze D, May MJ (1997) Cell proliferation and hair tip growth in the Arabidopsisroot are under mechanistically different forms of redox control. Proc Natl Acad Sci USA 94:2745–2750

    Article  PubMed  ADS  CAS  Google Scholar 

  • Schultz SG (1980) Basic principles of membrane transport. Cambridge University Press, Cambridge, xii and 144 pp

    Google Scholar 

  • Schunmann PH, Richardson AE, Smith FW, Delhaize E (2004) Characterization of promoter expression patterns derived from the Pht1 phosphate transporter genes of barley (Hordeum vulgareL.). J Exp Bot 55:855–865

    Article  PubMed  CAS  Google Scholar 

  • Schikora A, Schmidt W (2001) Acclimative changes in root epidermal cell fate in response to Fe and P deficiency: a specific role for auxin? Protoplasma 218:67–75

    Article  PubMed  CAS  Google Scholar 

  • Shabala S, Lew RR (2002) Turgor regulation in osmotically stressed Arabidopsis thalianaepidermal root cells: Direct support for the role of inorganic ion uptake as revealed by concurrent flux and cell turgor measurements. Plant Physiol 129:290–299

    Article  PubMed  CAS  Google Scholar 

  • Shin R, Berg RH, Schachtman DP (2005) Reactive oxygen species and root hairs in Arabidopsis root response to nitrogen, phosphorus and potassium deficiency. Plant Cell Physiol 46:1350–1357

    Article  PubMed  CAS  Google Scholar 

  • Silverman FP, Assiamah AA, Bush DS (1998) Membrane transport and cytokinin action in root hairs of Medicago sativa. Planta 205:23–31

    Article  CAS  Google Scholar 

  • Smith FW, Ealing PM, Hawkesford MJ, Clarkson DT (1995) Plant members of a family of sulfate transporters reveal functional subtypes. Proc Natl Acad Sci USA 92:9373–9377

    Article  PubMed  ADS  CAS  Google Scholar 

  • Sondergaard TE, Schulz A, Palmgren MG (2004) Energization of transport processes in plants. Roles of the plasma membrane H+ -ATPase. Plant Physiol 136:2475–2482

    Article  PubMed  CAS  Google Scholar 

  • Thomas RC (1978) Ion-sensitive intracellular microelectrodes. How to make and use them. Academic, London, xiii and 110 pp

    Google Scholar 

  • Torralba S, Heath IB (2001)[B2] Cytoskeletal and Ca2+regulation of hyphal tip growth and initiation. Curr Top Dev Biol 51:135–187

    PubMed  CAS  Google Scholar 

  • Tretyn A, Wagner G, Felle HH (1991) Signal transduction in Sinapis albaroot hairs: Auxins as external messengers. J Plant Physiol 139:187–193

    CAS  Google Scholar 

  • Tsay Y-F, Schroeder JI, Feldmann KA, Crawford NM (1993) The herbicide sensitivity gene CHL1 of Arabidopsisencodes a nitrate-inducible nitrate transporter. Cell 72: 705–713

    Article  PubMed  CAS  Google Scholar 

  • Tsuchiya K, Wang W, Giebisch G, Welling PA (1992) ATP is a coupling modulator of parallel Na,K-ATPase-K-channel activity in the renal proximal tubule. Proc Natl Acad Sci USA 89:6418–6422

    Article  PubMed  ADS  CAS  Google Scholar 

  • Ullrich CI, Novacky AJ (1990) Extra- and intracellular pH and membrane potential changes induced by K+, Cl−, , and uptake and fusicoccin in root hairs of Limnobium stoloniferum. Plant Physiol 94:1561–1567

    Article  PubMed  CAS  Google Scholar 

  • Volkov AG (2006) Plant Electrophysiology. Theory and Methods. Springer, Berlin Heidelberg New York , xxi and 508 pp

    Google Scholar 

  • Wang R, Crawford NM (1996) Genetic identification of a gene involved in constitutive, high-affinity nitrate transport in higher plants. Proc Natl Acad Sci USA 93:9297–9301

    Article  PubMed  ADS  CAS  Google Scholar 

  • Wang R, Liu D, Crawford NM (1998) The ArabidopsisCHL1 protein plays a major role in high-affinity nitrate uptake. Proc Natl Acad Sci USA 95:15134–15139

    Article  PubMed  ADS  CAS  Google Scholar 

  • Xu J, Li H-D, Chen L-Q, Wang Y, Liu L-L, He L, Wu W-H (2006) A protein kinase, interacting with two calcineurin B-like proteins, regulates K+transporter AKT1 in Arabidopsis. Cell 125:1347–1360

    Article  PubMed  CAS  Google Scholar 

  • Yoshimoto N, Takahashi H, Smith FW, Yamaya T, Saito K (2002) Two distinct high-affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsisroots. Plant J 29:465–473

    Article  PubMed  CAS  Google Scholar 

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    R. R. Lew

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Lew, R.R. (2008). Root Hair Electrophysiology. In: Plant Cell Monographs. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_2008_9

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