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NGF Uptake and Retrograde Signaling Mechanisms in Sympathetic Neurons in Compartmented Cultures

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Cell Biology of the Axon

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 48))

Abstract

Many neurons depend for their survival on retrograde signals to their cell bodies generated by nerve growth factor (NGF) or other neurotrophins at their axon terminals. Apoptosis resulting from the loss of retrograde NGF signaling contributes to the elimination of excess and misconnected neurons during development and to the death of neurons during the course of neurodegenerative diseases. Possible mechanisms of retrograde signaling include (1) retrograde transport of signaling endosomes, carrying NGF bound to activated TrkA, (2) retrograde transport of signaling molecules downstream of TrkA, and (3) retrograde propagation of a phosphorylation signal without transport of signaling molecules. Evidence is also described, which indicates that two or more retrograde signaling mechanisms exist to regulate neuronal survival, including recent evidence that withdrawal of NGF from distal axons produces a retrograde apoptotic signal, which is transported to the cell bodies, where it initiates the apoptotic program, leading to the death of the neuron.

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Acknowledgments

Our investigations of retrograde NGF signaling in compartmented cultures represent the work of four excellent Ph.D. students: Daren Ure, Donna Senger, Bronwyn MacInnis, and Sue-Ann Mok. Excellent technical support was provided by Karen Lund, Grace Martin, Norma Jean Valli, and Megan Blacker. Financial support has been provided by the Canadian Institutes of Medical Research, The Alberta Heritage Foundation, The Alberta Paraplegic Foundation, and the Rick Hansen Man in Motion Foundation.

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  1. Department of Cell Biology, University of Alberta, Edmonton, AB T6G 2H7, Canada

    Robert B. Campenot

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  1. Robert B. Campenot
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Correspondence to Robert B. Campenot .

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  1. Summer Hill Lane 70, Williamsville, 14221, U.S.A.

    Edward Koenig

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© 2009 Springer-Verlag Berlin Heidelberg

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Campenot, R.B. (2009). NGF Uptake and Retrograde Signaling Mechanisms in Sympathetic Neurons in Compartmented Cultures. In: Koenig, E. (eds) Cell Biology of the Axon. Results and Problems in Cell Differentiation, vol 48. Springer, Berlin, Heidelberg. https://doi.org/10.1007/400_2009_7

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