Abstract
Fluorescence correlation spectroscopy (FCS) is a detection technique for molecular parameters, such as concentration, diffusion constant and interaction in solution with single molecule level. FCS consists of a confocal optics with a well focused laser beam and detects fluorescent fluctuation that is caused by the diffusion of fluorescently labeled molecules through a tiny open volume element (< one femtoliter). Measured fluctuation can be used to calculate the lateral diffusion coefficient and thus the size of the molecule. We propose FCS as a newly method for the detection of prion diseases.
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© 2005 Springer-Verlag Tokyo
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Fujii, F., Sakata, H., Ueno, M., Yanagiya, T., Tamura, M., Kinjo, M. (2005). Detection of recombinant bovine prion protein by fluorescence correlation spectroscopy. In: Kitamoto, T. (eds) Prions. Springer, Tokyo. https://doi.org/10.1007/4-431-29402-3_16
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DOI: https://doi.org/10.1007/4-431-29402-3_16
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-25539-0
Online ISBN: 978-4-431-29402-3
eBook Packages: MedicineMedicine (R0)