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Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS)

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Metabolomics

7. Conclusion

Metabolic profiling by FT-ICR-MS and ESI-Q-TOF-MS is an effective method to capture a rough image of metabolism. It should be performed after the accuracy is confirmed by using the authentic sample after an elaborate calibration is done, when an individual metabolite is identified from the accurate mass for FT-ICR-MS. Moreover, when ESI-Q-TOF-MS is used, the CID spectrum should also be measured, and compared with that of the authentic sample.

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References

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© 2005 Springer-Verlag Tokyo

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Hirayama, K. (2005). Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS). In: Tomita, M., Nishioka, T. (eds) Metabolomics. Springer, Tokyo. https://doi.org/10.1007/4-431-28055-3_6

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