Abstract
Standard analytical ultracentrifugation is able to generate a significant hydrostatic pressure at the bottom of a spinning cell. In effect, a pressure gradient is created along its axis which, at maximum angular speeds, can reach nearly 50 MPa (around 500 atm). This pressure is high enough to alter the properties of some specially sensitive biological systems. Although this phenomenon can lead to important mistakes if the possible effects of hydrostatic pressure are not duly considered, it is possible to study the effect of the moderately high pressures generated in ultracentrifugation on biological systems of interest, such as macromolecular complexes or protein assemblies.
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References
Josephs R, Harrington WF (1967) Proc Natl Acad Sci USA 58: 1587
Van Diggelen OP, Oostrom H, Bosch L (1973) Eur J Biochem 39: 511
Poto EM, Wood HG (1977) Biochemistry 16: 1949
Marcum JM, Borisy GG (1978) J Biol Chem 253: 2852
Morel JE, Garrigos M (1982) Biochemistry 21: 2679
Harrington WF, Kegeles G (1973) Methods Enzymol 27: 306
Wattiaux R, Wattiaux-De Coninck S, Ronveaux-Dupal MF (1971) Arch Int Physiol Biochim 79: 214
Rosin MP, Zimmerman AM (1977) Mutat Res 44: 207
Wattiaux-De Coninck S, Dubois F, Wattiaux R (1977) Biochim Biophys Acta 471:421
Champeil P, Buschlen S, Guillain F (1981) Biochemistry 20: 1520
Halle D, Yedgar S (1988) Biophys J 54: 393
Weber G, Drickamer HG (1983) Q Rev Biophys 16: 89
Silva JL, Weber G (1993) Annu Rev Phys Chem 44: 89
Cioni P, Strambini GB (1994) J Mol Biol 242: 291
Atkins WM, Dietze EC, Ibarra C (1997) Protein Sci 6: 873
Jung C, Hui Bon Hoa G, Davydov D, Gill E, Heremans K (1995) Eur J Biochem 233: 600
Prevelige PE Jr, King J, Silva, JL (1994) Biophys J 66: 1631
Pin S, Royer CA, Gratton E, Alpert B, Weber G (1990) Biochemistry 29: 9194
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© 1999 Springer-Verlag
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Molina-Garcia, A.D. (1999). Hydrostatic pressure in ultracentrifugation. In: Cölfen, H. (eds) Analytical Ultracentrifugation V. Progress in Colloid and Polymer Science, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-48703-4_9
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DOI: https://doi.org/10.1007/3-540-48703-4_9
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