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Plant Metabolomics Strategies Based upon Quadrupole Time of Flight Mass Spectrometry (QTOF-MS)

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Plant Metabolomics

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 57))

5 Conclusions and Future Prospects

The examples presented in this chapter clearly underline the versatility of hybrid TOF mass spectrometers, and their capabilities with regard to metabolic profiling, structure elucidation and compound identification, using accurate mass determinations and MS/MS fragmentation. The high sensitivity and mass resolution allows the rapid screening of complex plant extracts by DFI, suitable for semi quantitative high throughput (pre)screening. More detailed analysis is possible when MS detection is preceded by applying separation technologies such as LC. Data processing and efficient data handling are becoming more and more the bottleneck in the process, especially when high through put screening is required and the currently available bioinformatics tools are inadequate. Another bottleneck is the low number of available reference compounds needed for definitive identification of differentially accumulating components. Key developments for the near future will therefore have to be made in these areas if true plant metabolomics strategies are to become routine. With better software and more easily mined databases we will be best equipped for the identification of the large numbers of the highly chemically diverse components typically present in complex plant extracts.

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Author information

Authors and Affiliations

  1. Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    H. A. Verhoeven, C. H. Ric de Vos, R. J. Bino & R. D. Hall

  2. Centre for BioSystems Genomics, P.O. Box 98, 6700 AB, Wageningen, The Netherlands

    H. A. Verhoeven, C. H. Ric de Vos, R. J. Bino & R. D. Hall

Authors
  1. H. A. Verhoeven
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  2. C. H. Ric de Vos
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  3. R. J. Bino
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  4. R. D. Hall
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Editor information

Editors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba, 263-8522, Japan

    Kazuki Saito

  2. RIKEN Plant Science Center, Yokohama, 230-0045, Japan

    Kazuki Saito

  3. Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

    Richard A. Dixon

  4. Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Golm, Germany

    Lothar Willmitzer

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© 2006 Springer-Verlag Berlin Heidelberg

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Verhoeven, H.A., Ric de Vos, C.H., Bino, R.J., Hall, R.D. (2006). Plant Metabolomics Strategies Based upon Quadrupole Time of Flight Mass Spectrometry (QTOF-MS). In: Saito, K., Dixon, R.A., Willmitzer, L. (eds) Plant Metabolomics. Biotechnology in Agriculture and Forestry, vol 57. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-29782-0_3

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