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Modern NMR Methodology pp 97–119Cite as

TROSY NMR Spectroscopy of Large Soluble Proteins

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Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 335))

Abstract

Solution nuclear magnetic resonance spectroscopy is usually only used to study proteins with molecular weight not exceeding about 50 kDa. This size limit has been lifted significantly in recent years, thanks to the development of labelling methods and the application of transverse-relaxation optimized spectroscopy (TROSY). In particular, methyl-specific labelling and methyl-TROSY have been shown to be effective for supramolecular systems as large as about 1 MDa. In this chapter we review the available methods for labelling different kinds of methyl groups and the assignment strategies in very large protein systems. Several proteins are selected as examples to show how NMR helps to reveal the details of structure, interaction and dynamics of these proteins.

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Abbreviations

ATCase:

Aspartate transcarbamoylase

CSA:

Chemical shift anisotropy

HMQC:

Heteronuclear multiple-quantum correlation

HSQC:

Heteronuclear single-quantum correlation

NMR:

Nuclear magnetic resonance

NOE:

Nuclear Overhauser effect

PCS:

Pseudocontact shift

PRE:

Paramagnetic relaxation enhancement

RDC:

Residual dipolar coupling

TROSY:

Transverse relaxation optimized spectroscopy

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Authors and Affiliations

  1. Division of Molecular Biosciences, Imperial College London, South Kensington, London, SW7 2AZ, UK

    Yingqi Xu & Stephen Matthews

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  1. Yingqi Xu
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  2. Stephen Matthews
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Correspondence to Stephen Matthews .

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Editors and Affiliations

  1. , ICS-6 / Strukturbiologie und Biophysik, Heinrich-Heine-Universität Düsseldorf/ F, Jülich, Jülich, 52425, Germany

    Henrike Heise

  2. , Division of Molecular Biosciences, Imperial College London, South Kensington, London, SW72AZ, United Kingdom

    Stephen Matthews

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Xu, Y., Matthews, S. (2011). TROSY NMR Spectroscopy of Large Soluble Proteins. In: Heise, H., Matthews, S. (eds) Modern NMR Methodology. Topics in Current Chemistry, vol 335. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_228

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