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Novel Electrochemical DNA Biosensors as Tools for Investigation and Detection of DNA Damage

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Trends in Bioelectroanalysis

Part of the book series: Bioanalytical Reviews ((BIOREV,volume 6))

Abstract

Supramolecular interactions of various organic xenobiotic compounds with deoxyribonucleic acid (DNA) are among the most important aspects of biological studies in clinical analysis, drug discovery, and pharmaceutical development processes. In recent years, there has been a growing interest in the electrochemical investigation of interactions between a studied analyte and DNA. Observing the pre- and post-electrochemical signals of DNA or monitoring its interaction with xenobiotics provides good evidence for the interaction mechanism to be elucidated. Such interaction can also be used for sensitive determination of these compounds. This short review summarizes our results obtained during the last 5 years in the field of novel electrochemical DNA biosensors utilizing carbon-based transducers as substrates for immobilization of DNA. It should provide evidence that the electrochemical approach (employing simple, fast, sensitive, and inexpensive DNA biosensors as tools for investigation and detection of DNA damage) brings new insight into human health protection or rational drug design and leads to further understanding of the interaction mechanism between xenobiotic compounds and DNA.

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Abbreviations

AA:

2-Aminoanthracene

Ag/AgCl:

Silver/silver chloride reference electrode

CFE:

Microcrystalline natural graphite–polystyrene composite film-modified electrode

CV:

Cyclic voltammetry

DNA:

Deoxyribonucleic acid

DPV:

Differential pulse voltammetry

dsDNA:

Double-stranded deoxyribonucleic acid

EIS:

Electrochemical impedance spectroscopy

GCE:

Glassy carbon electrode

IARC:

International Agency for Research on Cancer

NTMA:

4-Nitro-3-(trifluoromethyl)aniline

PB:

Phosphate buffer

phen:

1,10-Phenanthroline

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

RSS:

Reactive sulfur species

SPCE:

Screen-printed carbon electrode

SWCNT:

Single-walled carbon nanotube

SWV:

Square-wave voltammetry

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Acknowledgments

This publication originated in the framework of the Specific University Research (SVV). V.V. thanks the Grant Agency of the Czech Republic (Project GP13-23337P), and A.H. thanks the Grant Agency of the Charles University in Prague (Project GAUK 430214/2014/B-CH/PrF) for the financial support.

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  1. Charles University in Prague, Faculty of Science, University Research Centre UNCE “Supramolecular Chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 12843, Prague 2, Czech Republic

    Vlastimil Vyskočil & Andrea Hájková

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  1. Vlastimil Vyskočil
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  2. Andrea Hájková
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Correspondence to Vlastimil Vyskočil .

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  1. Institute of Analytical Chemistry, University of Regensburg, Regensburg, Germany

    Frank-Michael Matysik

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Vyskočil, V., Hájková, A. (2016). Novel Electrochemical DNA Biosensors as Tools for Investigation and Detection of DNA Damage. In: Matysik, FM. (eds) Trends in Bioelectroanalysis. Bioanalytical Reviews, vol 6. Springer, Cham. https://doi.org/10.1007/11663_2015_5002

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