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Measuring Biological Impacts of Nanomaterials pp 1–29Cite as

Characterization of Nanoparticles Under Physiological Conditions

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Part of the book series: Bioanalytical Reviews ((BIOREV,volume 5))

Abstract

In this article, well-established characterization methods for nanoparticles (NPs) are discussed, in particular their application under physiological conditions. The impact of different media, mimicking physiological conditions, on NP stability in terms of physiological ionic strength and formation of the NP–protein corona is described. In order to characterize NPs under physiological conditions, we distinguish between scattering and correlation methods, microscopy-based methods, and methods based on hydrodynamic separation. Features and limitations of relevant characterization methods are reviewed, as well as challenges arising in physiological media from enhanced aggregation tendency and the presence of proteins. We conclude that no available method for NP characterization in physiological media is able to describe the colloidal system completely and satisfactory. On the contrary, combining well-chosen analytical methods by taking benefits and disadvantages into account may provide detailed characterization results.

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Authors and Affiliations

  1. Fraunhofer ICT-IMM, Carl-Zeiss-Straße 18-20, 55129, Mainz, Germany

    K. A. Eslahian, T. Lang, C. Bantz, R. Keller, R. Sperling & M. Maskos

  2. Molecular and Cellular Oncology/Mainz Screening Center, University Medical Center of Mainz, Langenbeckstraße 1, 55101, Mainz, Germany

    D. Docter & R. Stauber

Authors
  1. K. A. Eslahian
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  2. T. Lang
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  3. C. Bantz
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  4. R. Keller
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  5. R. Sperling
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  6. D. Docter
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  7. R. Stauber
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  8. M. Maskos
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Corresponding author

Correspondence to M. Maskos .

Editor information

Editors and Affiliations

  1. University of Regensburg, Regensburg, Germany

    Joachim Wegener

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© 2014 Springer International Publishing Switzerland

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Eslahian, K.A. et al. (2014). Characterization of Nanoparticles Under Physiological Conditions. In: Wegener, J. (eds) Measuring Biological Impacts of Nanomaterials. Bioanalytical Reviews, vol 5. Springer, Cham. https://doi.org/10.1007/11663_2014_10

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